Pancreas performs both the endocrine and the exocrine function. The bulk of pancreas is composed of exocrine cells that produce digestion enzymes which are released into ducts to be emptied into the duodenum. The endocrine pancreas is composed of islets of Langerhans that produce hormones. At least five distinct endocrine cell types have been identified in the endocrine pancreas, each producing a different hormone. Beta cells produce insulin, which is considered as an anabolic hormone and regulates blood glucose levels and is also involved in fat and protein synthesis. Alpha cells produce glucagon that acts when blood glucose levels are low. Ductal cells of pancreas form the epithelial lining of the branched tubes that deliver digestive enzymes into the duodenum. They also secrete bicarbonate to neutralize acidic contents coming in from stomach. Duct cells may contribute up to 10% of all pancreatic cells. Acinar cells function as a unit of the exocrine pancreas and they synthesize, store, and secrete digestive enzymes (lipase, alpha amylase, trypsin, and chymotrypsin), which under normal physiological conditions are activated only after reaching the duodenum. Premature activation of these enzymes within pancreatic acinar cells can lead to acute pancreatitis. Clone DHIC5-4D9 can be used to detect duct cells in human pancreas and liver tissue. (Ref.: Dorrell, C., et al. (2008). Stem Cell Research (2008). 1(3); 183-194; Grapin-Botton, A (2005). Int. J. Biochem. Cell Biol. 37(3); 504-510; Leung, PS (2006). Int. J. Biochem. Cell Biol. 38(7); 1024-1030).

Human pancreas enriched in duct cells.

Anti-HPd3, clone DHIC5-4D9, Cat. No. MABS2040, is a mouse monoclonal antibody that detects pancreatic and hepatic ductal cells and has been tested for use in Immunohistochemistry (Paraffin).

Research Category
Signaling

Clone DHIC5-4D9 detects human pancreatic and liver duct cells.

Format: Purified

Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Immunohistochemistry (Paraffin) in human pancreas and human liver tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected HPd3 in human pancreas and human liver tissue sections.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.