Prostaglandin F2 receptor negative regulator (UniProt: Q9P2B2; also known as CD9 partner 1, CD9P1; Glu-Trp-Ile EWI motif-containing protein F; nEWI-F; Prostaglandin F2-alpha receptor regulatory protein; Prostaglandin F2-alpha receptor-associated protein; CD315) is encoded by the PTGFRN (also known as CD9P1, EWIF, FPRP, KIAA1436) gene (Gene ID 5738 ) in human. CD315 inhibits the binding of prostaglandin F2-alpha (PGF2-alpha) to its specific prostaglandin F2 alpha receptor, by decreasing the receptor number rather than the affinity constant. It is also reported to complex with 4-TM CD9 and CD81 and link them to the intracellular actin cytoskeleton. Its extracellular domain (ECD) contains six C2-type Ig-like domains (aa22-813) plus a Pro-Thr-Glu-Leu ER retention signal segment.

Clone 17H1 detects CD315 in A549 cells by Flow cytometry.

Epitope: extracellular domain

Mesothelioma cell-derived exosomes.

Flow Cytometry Analysis: 1:100 dilution of this antibody from a representative lot detected CD315 in one million U373 glioblastoma cells (Courtesy of Reinhard Zeidler, Helmholtz Zentrum Muenchen, Neuherberg, Germany in cooperation with Dr. A. Bankfalvi, Inst. für Pathologie Universitätsklinkum, Essen).

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected CD315 in Squamous cell carcinoma tissue (Courtesy of Reinhard Zeidler, Helmholtz Zentrum Muenchen, Neuherberg, Germany in cooperation with Dr. A. Bankfalvi, Inst. für Pathologie Universitätsklinkum, Essen).

Research Category
Cell Structure

This rat monoclonal Anti-CD315 antibody, clone 17H1, Cat. No. MABT883 is validated for use in Flow Cytometry and Immunohistochemistry for the detection of CD315.

Evaluated by Flow Cytometry in A549 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected CD315 in one million A549 cells.

98.55 kDa calculated

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.