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RADPKMAG-80K

MILLIPLEX® Rat Adipokine Panel - Metabolism Assay

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000

Key Documents

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species reactivity

rat

manufacturer/tradename

Milliplex®

assay range

accuracy: 94-119%
sensitivity: 0.3-17 pg/mL
(MinDC)
standard curve range: 0.7-3,000 pg/mL
(IL-1β, TNFα)
standard curve range: 12-50,000 pg/mL
(PAI-1)
standard curve range: 15-60,000 pg/mL
(IL-6, Leptin)
standard curve range: 24-100,000 pg/mL
(Insulin)
standard curve range: 9.8-40,000 pg/mL
(MCP-1)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

Quality Level

200

Related Categories

Application

  • Analytes: IL-1β, IL-6, Insulin, Leptin, MCP-1 (CCL2), PAI-1 (Total), TNFα
  • Recommended Sample Type: Rat serum or plasma
  • Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma
  • Assay Run Time: Overnight (16-18) hours at 2-8°C
  • Research Category: Metabolism
  • Research Subcategory: Endocrine; Obesity; Metabolic Disease

Biochem/physiol Actions

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

In the past, the role of white adipose tissue was thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism.

MILLIPLEX® Rat Adipokine Magnetic Bead Panel is a seven-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum and plasma samples: IL-1β, IL-6, Insulin, Leptin, MCP-1, PAI-1, and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism
The MILLIPLEX® MAP Rat Adipokine Magnetic Bead Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum or plasma samples: IL-1β, IL-6, Insulin, Leptin, MCP-1, PAI-1 Total, and TNFα.

Other Notes

Please contact Technical Service for linearity of dilution.
Rat Adipokine Standard, 1 vial, lyophilized

Rat Adipokine Quality Control 1, 1 vial, lyophilized

Rat Adipokine Quality Control 2, 1 vial, lyophilized

Assay Buffer, 1 bottle, 30 mL

Bead Diluent, 1 vial, 3.5 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea

Rat Adipokine Detection Antibodies, 1 bottle, 5.5 mL

Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL

Mixing Bottle, 1 bottle

Sample Plate, 96 well format, 1 plate

2 Foil Plate Sealers
Sensitivity: Please see kit protocol for individual assay sensitivities.

Packaging

Everything you need in a single kit.

Preparation Note

Recommended storage for kit components is 2 - 8°C.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Regulatory Information

新产品
This item has

Certificates of Analysis (COA)

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Lin Song et al.
Biology of reproduction, 102(3), 705-716 (2019-11-20)
Maternal high-fat (HF) diet negatively affects maternal metabolism and placental function. This study aimed to determine whether gestational exercise prevents the effect of HF diet on placental amino acid transporter expression and nutrient-sensing signaling and the fetal response. Pregnant Sprague-Dawley
Jaroslava Trnovska et al.
International journal of molecular sciences, 22(19) (2021-10-14)
(1) Background: empagliflozin, sodium-glucose co-transporter 2 (SGLT-2) inhibitor, is an effective antidiabetic agent with strong cardio- and nephroprotective properties. The mechanisms behind its cardio- and nephroprotection are still not fully clarified. (2) Methods: we used male hereditary hypertriglyceridemic (hHTG) rats
Maria Monica Michicotl-Meneses et al.
Nutrients, 13(8) (2021-08-28)
Pomegranate juice (Punica granatum) has been used since ancient times in traditional medicine (Unani Medicine, Ayurveda); its main compounds are anthocyanins and ellagic acid, which have anti-inflammatory, antioxidant, hepatoprotective, and cardiovascular health effects. The objective was to evaluate the effect
Zongbin Song et al.
Scientific reports, 7(1), 10350-10350 (2017-09-06)
Obesity is associated with increased risk for chronic pain. Basic mechanisms for this association are poorly understood. Using a milder version of a radicular pain model, local inflammation of the dorsal root ganglion (DRG), we observed marked increases in mechanical

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96 well Template Single
Protocol- RADPKMAG-80K
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