S7821-M
CpGenome Universal Methylated DNA
Enzymatically methylated human male genomic DNA to be used as a methylation-positive control for gene methylation studies.
Synonym(s):
Enzymatically methylated human male genomic DNA, Methylation-positive control
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32
Quality Level
form
liquid
species reactivity
human
manufacturer/tradename
Chemicon®
CpGenome
solubility
H2O: soluble at 20 °C
application(s)
genomic analysis
storage temp.
−20°C (−15°C to −25°C)
General description
CpGenome Universal Methylated DNA is enzymatically methylated human male genomic DNA. This product is intended for use with the CpGenome DNA Modification Kit (S7820) or with amplification primers. It can be used as a methylation-positive control for gene methylation studies.
Materials Provided:
Each vial contains 10 micrograms (100 μl) of human genomic DNA at a concentration of 0.1 μg/μl. Note: this DNA must first be bisulfite modified (S7820) to use a primer that is specific for the methylated form of the gene of interest.
Validation:
Methylation-specific PCR (MSP) was performed on the DNA prior to and post-enzymatic methylation. Sets of primers from the CpG WIZ p15 and E-cadherin Amplification Kits were used in the assay. A set of primers designed to anneal to unmethylated DNA and a second set designed to anneal to methylated sequences were used from each kit. The methylated primer sets generated products only after the DNA was methylated.
CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.
Materials Provided:
Each vial contains 10 micrograms (100 μl) of human genomic DNA at a concentration of 0.1 μg/μl. Note: this DNA must first be bisulfite modified (S7820) to use a primer that is specific for the methylated form of the gene of interest.
Validation:
Methylation-specific PCR (MSP) was performed on the DNA prior to and post-enzymatic methylation. Sets of primers from the CpG WIZ p15 and E-cadherin Amplification Kits were used in the assay. A set of primers designed to anneal to unmethylated DNA and a second set designed to anneal to methylated sequences were used from each kit. The methylated primer sets generated products only after the DNA was methylated.
CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.
Physical form
Liquid in TE (10mM Tris-HCL, 0.1mM EDTA) with no preservatives.
Preparation Note
Recommended Storage: -15°C to -25°C, in aliquots, protected from freeze-thaws for up to two years from date of receipt. Do not freeze and thaw the same -20°C aliquot more than 3X for best results. Multiple freeze thaws can fragment DNA. Storage at -70°C can be used for longer term storage if necessary; minimize multiple freeze thaws for best results.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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DNA hypermethylation and histone modifications downregulate the candidate tumor suppressor gene RRP22 on 22q12 in human gliomas.
Natalie Schmidt,Sonja Windmann,Guido Reifenberger,Markus J Riemenschneider
Brain Pathology null
Detection of epigenetic changes in fecal DNA as a molecular screening test for colorectal cancer: a feasibility study
Leung, Wai K, et al
Clinical Chemistry, 50, 2179-2182 (2004)
Shinobu Sato et al.
Analytica chimica acta, 578(1), 82-87 (2007-08-29)
Ferrocenylnaphthalene diimide (FND)-based electrochemical hybridization assay was applied to the detection of methylated cytosine of DNA using the products obtained after treatment with bisulfite followed by polymerase chain reaction (PCR), where unmethylated cytosine is converted to thymine and methylated one
Closed-tube PCR methods for locus-specific DNA methylation analysis.
Ida L M Candiloro,Thomas Mikeska,Alexander Dobrovic
Methods in Molecular Biology null
Capillary electrophoretic analysis of methylation status in CpG-rich regions by single-base extension of primers modified with N6-methoxy-2,6-diaminopurine.
Victoria L Boyd, Gerald Zon
Analytical biochemistry null
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