PMC42-LA Human Breast Cancer Cell Line

Human breast carcinoma cell lines are useful models to investigate the pathogenesis of neoplastic transformation and aid in the development of novel therapeutic strategies. Nonetheless, there are few well characterized cell lines derived from human mammary carcinomas and loss of breast-specific marker expression and the emergence of dedifferentiated phenotypes over extended culture often limit their use .

Initiated from a pleural effusion of a metastatic breast cancer patient, the human mesenchymal breast carcinoma cell line PMC42 (also known as PMC42ET), is unique in that these cells remained pleomorphic even after several years of sustained culture . Typically grown in monolayer, PMC42 continue to give rise to numerous morphologically different cell types of both secretory and myoepithelial characteristics and this retained ability to differentiate in vitro earned their designation as stem cell like .

PMC42-LA is a stable epithelial variant of the mesenchyme-like parental PMC42 /PMC42-ET cell line. PMC42-LA congregate into pavement epithelial sheets at high density, in which the cell-cell borders contain E-cadherin and -catenin, albeit 5-10% of cells do express the mesenchymal marker vimentin. When stimulated with epidermal growth factor (EGF), PMC42-LA cells undergo an epithelial-mesenchymal transition in which vimentin expression is upregulated and E-cadherin expression is reduced .

Research Category
Cancer

Oncology

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Cancer Cells

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

• Each vial contains ≥ 1X106 viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, C, and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.