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Merck
CN

ST1705

Anti-TOMM20 Mouse mAb (4F3)

liquid, clone 4F3, Calbiochem®

Synonym(s):

Anti-Translocase of Outer Mitochondrial Membrane 20

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About This Item

NACRES:
NA.43
UNSPSC Code:
12352203

Key Documents

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Product Name

Anti-TOMM20 Mouse mAb (4F3), liquid, clone 4F3, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

4F3, monoclonal

form

liquid

does not contain

preservative

species reactivity

human, rat, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(ELISA (see coments)
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (10 µg/mL)
Paraffin Sections (3 µg/mL))

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... TOMM20(9804)

Analysis Note

Negative Control
293T cells
Positive Control
HeLa cells, PC12 cells, NIH3T3 cells, Human small intestine tissue

Application






ELISA (see coments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (3 g/ml)

Disclaimer

Toxicity: Regulatory Review (Z)

General description

Purified mouse monoclonal antibody. Recognizes the ~12-16 kDa TOMM20 protein.
Recognizes the ~12-16 kDa TOMM20 protein in HeLa, PC12, and NIH3T3 cells and human small intestine tissue.
This Anti-TOMM20 Mouse mAb (4F3) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of TOMM20.

Immunogen

Full-length, recombinant, human TOMM20 (aa 1-146), expressed as a GST fusion protein

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Joe C Udeochu et al.
Nature neuroscience, 26(5), 737-750 (2023-04-25)
Pathological hallmarks of Alzheimer's disease (AD) precede clinical symptoms by years, indicating a period of cognitive resilience before the onset of dementia. Here, we report that activation of cyclic GMP-AMP synthase (cGAS) diminishes cognitive resilience by decreasing the neuronal transcriptional
Adam Z Spitz et al.
Nature communications, 12(1), 1134-1134 (2021-02-20)
The BCL-2 family protein BAX has essential activity in mitochondrial regulation of cell death. While BAX activity ensures tissue homeostasis, when dysregulated it contributes to aberrant cell death in several diseases. During cellular stress BAX is transformed from an inactive

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