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10378445001

Roche

Chromozym PK

90% (enzymatic), powder, pkg of 20 mg

Synonym(s):

C33H39N8O6COOCH3

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
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description

Molecular Formula: C33H39N8O6COOCH3

assay

90% (enzymatic)

form

powder

mol wt

Mr 702.9

packaging

pkg of 20 mg

manufacturer/tradename

Roche

storage temp.

room temp

General description

Chromozym® PK is a non-radioactive amidolytic chromogenic substrate mostly used in kinetic analysis of plasma kallikrein.

Application

Use Chromozym® PK as a substrate for the determination of serine proteases, specifically plasma kallikrein in citrated plasma.

Biochem/physiol Actions

Chromozym PK is cleaved by plasma kallikrein into a residual peptide and free 4-nitraniline, which is measured at 405 nm. The absorbance difference per minute is used for the determination of the kallikrein activity in U/ml.

Preparation Note

Activator: Mg2+ (Km = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn2+, Fe2+, Zn2+)
Working concentration: Approximately 0.5 mM
Storage conditions (working solution): 2 to 8 °C
An aqueous solution of Chromozym PK (1.3 mM) is stable for at least 4 weeks if stored at 2 to 8 °C.

Analysis Note

Contaminant: <0.5% free 4-nitraniline

Other Notes

Benzoyl-prolyl-phenalanyl-arginine-4-nitranilide-acetate
For life science research only. Not for use in diagnostic procedures.

Legal Information

Chromozym is a registered trademark of Pentapharm AG, Basel

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
23000920
93117420
78981420
46731821
38634020

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R Ito et al.
Clinical chemistry, 27(4), 586-593 (1981-04-01)
The amidolytic activity of activated kallikrein in plasma can be measured by use of the chromogenic substrate, S-2302 (H-D-Pro-Phe-Arg-pNA). Plasma prekallikrein was activated to kallikrein by exposure to 50 mg/L dextran sulfate in acetone/water (35/65 by vol) at 0 degrees

Global Trade Item Number

SKUGTIN
1037844500104061838670809