10378445001
Roche
Chromozym PK
90% (enzymatic), powder, pkg of 20 mg
Synonym(s):
C33H39N8O6COOCH3
description
Molecular Formula: C33H39N8O6COOCH3
Quality Level
Assay
90% (enzymatic)
form
powder
mol wt
Mr 702.9
packaging
pkg of 20 mg
manufacturer/tradename
Roche
storage temp.
room temp
Related Categories
General description
Chromozym® PK is a non-radioactive amidolytic chromogenic substrate mostly used in kinetic analysis of plasma kallikrein.
Application
Use Chromozym® PK as a substrate for the determination of serine proteases, specifically plasma kallikrein in citrated plasma.
Biochem/physiol Actions
Chromozym PK is cleaved by plasma kallikrein into a residual peptide and free 4-nitraniline, which is measured at 405 nm. The absorbance difference per minute is used for the determination of the kallikrein activity in U/ml.
Preparation Note
Activator: Mg2+ (Km = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn2+, Fe2+, Zn2+)
Working concentration: Approximately 0.5 mM
Storage conditions (working solution): 2 to 8 °C
An aqueous solution of Chromozym PK (1.3 mM) is stable for at least 4 weeks if stored at 2 to 8 °C.
Working concentration: Approximately 0.5 mM
Storage conditions (working solution): 2 to 8 °C
An aqueous solution of Chromozym PK (1.3 mM) is stable for at least 4 weeks if stored at 2 to 8 °C.
Analysis Note
Contaminant: <0.5% free 4-nitraniline
Other Notes
Benzoyl-prolyl-phenalanyl-arginine-4-nitranilide-acetate
For life science research only. Not for use in diagnostic procedures.
Legal Information
Chromozym is a registered trademark of Pentapharm AG, Basel
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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R Ito et al.
Clinical chemistry, 27(4), 586-593 (1981-04-01)
The amidolytic activity of activated kallikrein in plasma can be measured by use of the chromogenic substrate, S-2302 (H-D-Pro-Phe-Arg-pNA). Plasma prekallikrein was activated to kallikrein by exposure to 50 mg/L dextran sulfate in acetone/water (35/65 by vol) at 0 degrees
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