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About This Item
Empirical Formula (Hill Notation):
C14H28O6
CAS Number:
Molecular Weight:
292.37
PubChem Substance ID:
UNSPSC Code:
12352200
Beilstein/REAXYS Number:
84118
MDL number:
NACRES:
NA.54
SMILES string
CCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O
InChI
1S/C14H28O6/c1-2-3-4-5-6-7-8-19-14-13(18)12(17)11(16)10(9-15)20-14/h10-18H,2-9H2,1H3/t10-,11-,12+,13-,14-/m1/s1
InChI key
HEGSGKPQLMEBJL-RKQHYHRCSA-N
assay
99% (GC)
mol wt
micellar avg mol wt 25,000, 292.4
packaging
pkg of 10 g
manufacturer/tradename
Roche
aggregation number
84
technique(s)
dialysis: suitable
CMC
20-25 mM (20-25°C)
transition temp
cloud point >100 °C
shipped in
ambient
General description
1-O-n-Octyl-β-D-glucopyranoside, octylglucoside, is a detergent with an octyl chain as hydrophobic residue and glucose as hydrophilic part. It is a non-ionic, dialyzable, mild and non-denaturing detergent which is used for the solubilization, isolation and reconstitution of membrane proteins.
Application
N-Octylglucoside has been shown to increase the resolution of proteins in 2D gels. It is a mild and non-denaturing detergent for the solubilization and reconstitution of membrane proteins. N-Octylglucoside is easily removed by dialysis. N-Octylglucoside has been used in mass spectrometric immunoassay to homogenize MALDI (matrix assisted laser desorption/ionization) matrix draw and elution.
Non-ionic, dialyzable detergent for the solubilization and isolation of membrane proteins. Has been shown to increase the resolution of proteins in 2D gels.
Preparation Note
Working concentration: 46 mM
Working solution: Solubility: > 50% (w/v) in double-dist. water, Tris-HCl 0.05 mol/l; pH7.4 or K-phosphate buffer 0.1 mol/l, pH 7.0 at 25 °C.
Storage conditions (working solution): Stability in Solution
A stock solution is stable at 2 to 8 °C for approx. 3 days. We would expect a long term stability of 6-12 months if stored frozen in portions at -15 to -25 °C, provided bacterial contamination is avoided.
Working solution: Solubility: > 50% (w/v) in double-dist. water, Tris-HCl 0.05 mol/l; pH7.4 or K-phosphate buffer 0.1 mol/l, pH 7.0 at 25 °C.
Storage conditions (working solution): Stability in Solution
A stock solution is stable at 2 to 8 °C for approx. 3 days. We would expect a long term stability of 6-12 months if stored frozen in portions at -15 to -25 °C, provided bacterial contamination is avoided.
Store at 15 to 25 °C. (Store dry!)
Analysis Note
Purity: 99% (from C), homogeneous (GC)
Ease of removal*: ++
CMC**: 14.5 mM at +25°C
Formula: C14H28O6
Ease of removal*: ++
CMC**: 14.5 mM at +25°C
Formula: C14H28O6
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
涉药品监管产品
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W A Petri et al.
The Journal of biological chemistry, 254(11), 4313-4316 (1979-06-10)
The glycoprotein of vesicular stomatitis (VS) virus was selectively liberated from the virion membrane by the dialyzable nonionic detergent, beta-D-octylglucoside. The isolated viral glycoprotein could be rendered virtually free of phospholipid and detergent, under which conditions it formed tail-to-tail glycoprotein
Howard R Mellor et al.
The Biochemical journal, 374(Pt 2), 307-314 (2003-05-29)
The N-alkyl moiety of N-alkylated imino sugars is crucial for therapeutic activities of these compounds as inhibitors of glycosphingolipid (GSL) biosynthesis and as antivirals. The improved potency afforded by a long N-alkyl moiety is coincident with increased compound-induced cytotoxicity. Therefore
Olgica Trenchevska et al.
Journal of proteome research, 9(11), 5969-5973 (2010-09-09)
Protein biomarkers are essential in assessing pathogenic processes. The impetus for finding new biomarkers has been accelerated by the arrival of the "omics" technologies. However, equally important is the rediscovery of existing biomarkers with these new approaches as novel variants
Olgica Trenchevska et al.
Proteome science, 9(1), 19-19 (2011-04-12)
Post-translational modifications and genetic variations give rise to protein variants that significantly increase the complexity of the human proteome. Modified proteins also play an important role in biological processes. While sandwich immunoassays are routinely used to determine protein concentrations, they
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