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11333054001

Roche

Digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid-N-hydroxysuccinimide ester

Name: Digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid-N-hydroxysuccinimide ester
Brand: ROCHE

>90% (elementary analysis), pkg of 5 mg, powder, pH range 2-13

Synonym(s):

Digoxigenin NHS-ester, dig-nhs-ester, ε-(Digoxigenin-3-0-acetamido)caproic acid N-hydroxysuccinimide ester, DIG NHS

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About This Item

Empirical Formula (Hill Notation):

C₃₅H₅₀N₂O₁₀

CAS Number:

129273-26-3

Molecular Weight:

658.78

UNSPSC Code:

41105600

PubChem Substance ID:

329749387

MDL number:

MFCD02093394

NACRES:

NA.54

Assay:

>90% (elementary analysis)

Form:

powder

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Properties

Quality Level

100

assay

>90% (elementary analysis)

form

powder

mol wt

M_r 658.8

packaging

pkg of 5 mg

manufacturer/tradename

Roche

pH range

2-13

solubility

DMF: soluble, DMSO: soluble, ethanol: soluble

storage temp.

−20°C

SMILES string

[H][C@]12CC[C@]3([H])[C@]([H])(C[C@@H](O)[C@]4(C)[C@H](CC[C@]34O)C5=CC(=O)OC5)[C@@]1(C)CC[C@@H](C2)OCC(=O)NCCCCCC(=O)ON6C(=O)CCC6=O

InChI

1S/C35H50N2O10/c1-33-13-11-23(45-20-28(39)36-15-5-3-4-6-31(42)47-37-29(40)9-10-30(37)41)17-22(33)7-8-25-26(33)18-27(38)34(2)24(12-14-35(25,34)44)21-16-32(43)46-19-21/h16,22-27,38,44H,3-15,17-20H2,1-2H3,(H,36,39)/t22-,23+,24-,25-,26+,27-,33+,34+,35+/m1/s1

InChI key

KHNDABJZSPPYLE-FUGFVFQCSA-N

Description

General description

Chemical reagent for the labeling of proteins and oligonucleotides. One reactive group is able to interact with NH₂-groups (of proteins or oligonucleotides), another reactive group (Digoxigenin) reacts with Anti-Digoxigenin and thus can be detected.

Application

Digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid-N-hydroxysuccinimide ester has been used to label the sequence (III) for the linker molecule sequence (VIII).

Biochem/physiol Actions

Digoxigenin, a non-radioactive immune-tag is mainly used for labeling proteins and in situ hybridization.

The activated ester reacts with amino groups under mild conditions. The digoxigenin moiety can be introduced into proteins or 5′-amino substituted oligonucleotides.

Preparation Note

Working concentration: 1 to 5 mM
Working solution: Preparation of working solution

Under stirring 0.327 mg digoxigenin-3-O-methylcarbonyl- ε-aminocaproic acid -N-hydroxysuccinimide ester is dissolved in 8.18 μl DMSO or ethanol.