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About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
description
Disodium 2-chloro-5-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo[3.3.1.13,7 ]decan}-4-yl)-1-phenyl phosphate
Quality Level
Assay
>98%
form
solution
packaging
bottle of 2 × 50 mL ([0.25 mM])
manufacturer/tradename
Roche
technique(s)
Northern blotting: suitable
Southern blotting: suitable
dot blot: suitable
storage temp.
2-8°C
Application
Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots:
- Southern blot
- Northern blot
- Dot blot
- Colony and plaque hybridization
- Gel shift assays
Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots:
- Southern blot
- Northern blot
- Dot blot
- Colony and plaque hybridization
- Gel shift assays
- Chemiluminescent detection.
Features and Benefits
Contents
0.25 mM solution (0.124 mg/ml), ready-to-use, colorless solution
0.25 mM solution (0.124 mg/ml), ready-to-use, colorless solution
CDP-Star is a chemiluminescent substrate for alkaline phosphatase that enables fast, ultra-sensitive detection of biomolecules by producing extremely rapid visible light. Light emission is recorded on X-ray film or on luminescence imager systems.
- Save time with a fast, ultra-sensitive substrate.
- Easily strip and reprobe membranes.
- Take multiple exposures of up to two days for each experiment.
Preparation Note
Storage conditions (working solution): Washing Buffer: 15 to 25 °C, stable
Maleic Acid Buffer: 15 to 25 °C, stable
Detection Buffer: 15 to 25 °C, stable
Blocking Solution: always prepare freshly
Antibody Solution: 2 to 8 °C
Maleic Acid Buffer: 15 to 25 °C, stable
Detection Buffer: 15 to 25 °C, stable
Blocking Solution: always prepare freshly
Antibody Solution: 2 to 8 °C
Other Notes
- First exposure to X-ray film should be 5-15 minutes. Examine film and then determine other exposure times based on initial result.
- No preincubation step required.
- Apply substrate quickly to avoid gray shadows from substrate drops and drying of membrane (leads to uneven, high background).
- Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
- Exposure times are shorter than with CSPD.
- Do not use nitrocellulose membranes.
For life science research only. Not for use in diagnostic procedures.
Legal Information
CDP-Star is a registered trademark of Tropix, Inc.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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