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DNTPM-RO

Roche

PCR Nucleotide Mix

solution, sufficient for 500 reactions (11581295001), sufficient for 5,000 reactions (11814362001), sufficient for 2,500 reactions (04638956001)

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grade

Molecular Biology

Quality Level

100

Assay

99.8% (sum of 4 peaks, HPLC)

form

solution

usage

sufficient for 2,500 reactions (04638956001)
 sufficient for 5,000 reactions (11814362001)
 sufficient for 500 reactions (11581295001)

packaging

pkg of 10 × 200 μL (11814362001 [10mM, each])
pkg of 200 μL (11581295001 [10 mM, each])
pkg of 5 × 200 μL (04638956001 [10 mM, each])

manufacturer/tradename

Roche

concentration

10 mM

impurities

DNase, none detected
 RNase, none detected

color

colorless

solubility

water: miscible

storage temp.

−20°C

Related Categories

General description

PCR Nucleotide Mix is a clear, colorless solution of the sodium salts of dATP, dCTP, dGTP, and dTTP, each at a concentration of 10 mM in Water, PCR Grade. This nucleotide mixture can be added directly to polymerase chain reactions.

Application

  • The PCR Nucleotide Mix is optimized for use in all types of amplification reactions and primer-extension reactions: PCR
  • RT-PCR
  • cDNA synthesis
  • Primer extension

Features and Benefits

The PCR Nucleotide Mix can be added directly to amplification reactions. PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity.

Improve yield and performance
  • Choose a convenient ready-to-use mix of all 4 nucleotides.
  • Achieve the highest PCR and RT-PCR sensitivity.
  • Insist on highest purity (>99%) nucleotides.

Packaging

1 set containing 4 ready-to-use mixes

Quality

Function Testing in PCR
Function Testing in RT-PCR
Absence of Contaminating Deoxyribonucleases/Nicking Activities according to the current Quality Control procedures.
Absence of Contaminating Ribonucleases according to the current Quality Control procedures.

Preparation Note

Activator: Mg2+
Working solution: If required, the solutions can be diluted with distilled autoclaved water.
Storage conditions (working solution): It will withstand 50 freeze/thaw cycles.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description
  • dATP, PCR Grade, sodium salt 10 mM
  • dCTP, PCR Grade, sodium salt 10 mM
  • dGTP, PCR Grade, sodium salt 10 mM
  • dTTP, PCR Grade, sodium salt 10 mM

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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Weimin Liu et al.
Protocol exchange, 2010 (2010-09-28)
Conventional PCR followed by molecular cloning and sequencing of amplified products is commonly used to test clinical specimens for target sequences of interest, such as viral, bacterial or parasite nucleic acids. However, this approach has serious limitations when used to
Tania Nolan et al.
Nature protocols, 1(3), 1559-1582 (2007-04-05)
The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the evident requirement for quantitative data analysis in molecular medicine, biotechnology, microbiology and diagnostics and has become the method of choice for the quantification of mRNA. Although it is often described

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