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Merck
CN

13-2260

NN

CP

Synonym(s):

Calconcarboxylic acid, Cal-Red™, 2-Hydroxy-1-(2-hydroxy-4-sulfo-1-naphthylazo)-3-naphthoic acid, 3-Hydroxy-4-(2-hydroxy-4-sulfo-1-naphthylazo)naphthalene-2-carboxylic acid, Patton and Reeder’s reagent

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About This Item

Empirical Formula (Hill Notation):
C21H14N2O7S
CAS Number:
3737-95-9
Molecular Weight:
438.41
PubChem Substance ID:
329750106
UNSPSC Code:
12171500
Beilstein/REAXYS Number:
727492
MDL number:
MFCD00004078
Grade:
CP

Key Documents

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SMILES string

OC(=O)c1cc2ccccc2c(N=Nc3c(O)cc(c4ccccc34)S(O)(=O)=O)c1O

InChI

1S/C21H14N2O7S/c24-16-10-17(31(28,29)30)13-7-3-4-8-14(13)18(16)22-23-19-12-6-2-1-5-11(12)9-15(20(19)25)21(26)27/h1-10,24-25H,(H,26,27)(H,28,29,30)/b23-22-

InChI key

MVQBFZXBLLMXGS-FCQUAONHSA-N

grade

CP

form

powder

availability

available only in Japan

concentration

~0.5%

dilution

(for analytical testing)

Other Notes

1:200 diluted with potassium sulfate

Legal Information

Cal-Red is a trademark of Sigma-Aldrich Co. LLC

Storage Class

13 - Non Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Li-Tai Jin et al.
Electrophoresis, 25(15), 2494-2500 (2004-08-10)
A highly sensitive silver staining method for detecting proteins in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was developed. It is based on the silver nitrate staining method but also employs an azo dye, calconcarboxylic acid (NN), as a silver-ion sensitizer.
Y Su et al.
The journal of physical chemistry. B, 117(6), 1563-1570 (2012-06-06)
The electrophoretic deposition (EPD) method has been developed for the fabrication of MnO(2)-multiwalled carbon nanotube (MWCNT) films for application in electrochemical supercapacitors (ESs). For MWCNT applications, which depend on electrical conductivity, it is challenging to achieve dispersion and EPD of
D W Jung et al.
Electrophoresis, 19(14), 2412-2415 (1998-11-20)
We have developed a new mixed-dye protein staining method that is simple, rapid, and sensitive. A freshly prepared mixture of calconcarboxylic acid (NN, 0.02%) and rhodamine B (RB, 0.04%) in 40% methanol/7% acetic acid, was used as a staining solution.
Li-Tai Jin et al.
Electrophoresis, 25(15), 2429-2438 (2004-08-10)
Since 1993, we have studied visible organic dye stains for protein or DNA to improve methodologies and developed the counterion dye staining method. The method employs two oppositely charged dyes that form an ion-pair complex in the staining solution. The
H Y Hong et al.
Analytical biochemistry, 214(1), 96-99 (1993-10-01)
We describe here a protein staining method in polyacrylamide gels with a new staining dye, 1-(2-hydroxy-4-sulfo-1-naphthylazo)-2-hydroxy-3-naphthoic acid (calconcarboxylic acid, NN). This method can be performed by both simultaneous and postelectrophoretic staining techniques. Simultaneous staining using 0.01% of NN in upper
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