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Sigma-Aldrich

SYBR® Green II RNA gel stain

greener alternative

10,000 × in DMSO

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Synonym(s):
RNA gel dye, SYBR® RNA dye, safer gel stain
CAS Number:
172827-25-7
EC Number:
200-664-3
MDL number:
MFCD00284716
NACRES:
NA.52

usage

 mL sufficient for 100 mini-gels

Quality Level

100

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

concentration

10,000 × in DMSO

greener alternative category

Aligned,

storage temp.

−20°C

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General description

SYBR® Green II is a highly sensitive stain for post electrophoresis staining of RNA and ssDNA in agarose or polyacrylamide gels. SYBR® Green II is not selective for RNA staining but does exhibit a higher quantum yield when bound to RNA than to double stranded DNA.
Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has inherently safer chemistry, compared to the standard use of ethidium bromide for staining. For more information see publication found in related content.

Application

SYBR® Green II RNA gel stain has been used:

  • for the quantification of ribonucleic acid (RNA) in Escherichia coli
  • for staining 5′-ETS rRNA species separated on polyacrylamide gel prior to Northern blot analysis
  • to stain formaldehyde agarose gels to visualize RNA bands

Features and Benefits

  • Ultrasensitive stain for post-electrophoresis staining of RNA and ssDNA
  • Excited maximally at 497 nm with a secondary excitation peak at 254nm
  • The fluorescence emission of SYBR®Green II stained RNA is centered at 520 nm
  • Staining agarose/formaldehyde gels with SYBR Green II does not interfere with the transfer of RNA to membranes or subsequent hybridization in Northern blot analysis as long as 0.1% -0.3% SDS is included in prehybridization and hybridization buffers to remove the dye
  • Might facilitate the detection of viroid RNAs and multicopy cellular RNA species
  • More sensitive than ethidium bromide in applications that require extremely sensitive detection techniques such as single-strand conformation polymorphism (SSCP) analysis

Legal Information

SYBR is a registered trademark of Life Technologies

replaced by

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Michael Richmond et al.
Biochemistry, 50(12), 2298-2312 (2011-02-09)
In this work, we describe RapA-dependent polyadenylation of model RNA substrates and endogenous, RNA polymerase-associated nucleic acid fragments. We demonstrate that the Escherichia coli RNA polymerase obtained through the classic purification procedure carries endogenous RNA oligonucleotides, which, in the presence
Human ??defensin-1 and -2 expression in the gingiva of patients with specific periodontal diseases
S. Vardar-Sengul
Journal of Periodontal Research (2007)
Benjamin Lau et al.
Molecular cell, 81(2), 293-303 (2020-12-17)
Ribosome assembly is catalyzed by numerous trans-acting factors and coupled with irreversible pre-rRNA processing, driving the pathway toward mature ribosomal subunits. One decisive step early in this progression is removal of the 5' external transcribed spacer (5'-ETS), an RNA extension
Jingdong Cheng et al.
Science (New York, N.Y.), 369(6510), 1470-1476 (2020-09-19)
Production of small ribosomal subunits initially requires the formation of a 90S precursor followed by an enigmatic process of restructuring into the primordial pre-40S subunit. We elucidate this process by biochemical and cryo-electron microscopy analysis of intermediates along this pathway
Benjamin Lau et al.
Molecular cell, 81(2), 293-303 (2020-12-17)
Ribosome assembly is catalyzed by numerous trans-acting factors and coupled with irreversible pre-rRNA processing, driving the pathway toward mature ribosomal subunits. One decisive step early in this progression is removal of the 5' external transcribed spacer (5'-ETS), an RNA extension
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