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Fathead minnow (Pimephales promelas), 48-hr Acute, nonrenewal, 25°C, MHSF

Proficiency Testing Material


Quality Level


ampule of 2 × 2 mL


in water




matrix material

General description

This Whole Effluent Toxicity (WET) proficiency testing sample is produced in accordance with ISO/IEC 17043:2010. This product is produced according to TNI requirements. However, please note, this sample may include non-TNI analytes as well.

In Acute WET tests, Fathead minnow is employed as a test organism to determine its mortality upon exposure to the effluent for a short duration (48 h). This helps arrive at the lethally toxic concentrations of the effluent.

Supelco® is a global proficiency testing (PT) provider that manufactures best-in-class environmental and pharmaceutical PT products. Most water PT samples are available as concentrates or ready-to-use volumes giving the end-user flexibility in selecting samples that will fit their exact needs. Our PT program is designed to meet the quality assurance requirements in compliance with Discharge Monitoring Report - Quality Assurance Program (DMR-QA), thereby ensuring the analytical competence of your laboratory.

Analyte/s: Values of analytes vary lot to lot.

All information regarding the use of Fathead minnow (Pimephales promelas), 48-hr Acute, nonrenewal, 25 °C, MHSF proficiency testing standard can be found in the reporting packet which will be available to download at PT Portal.


Fathead minnow (Pimephales promelas), 48-hr Acute, nonrenewal, 25 °C, MHSF proficiency testing material is designed for use in Acute Whole Effluent Toxicity (WET) proficiency testing.

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For more information on this product, please contact our PT Service Team.

Features and Benefits

We have a vast portfolio of environmental PT schemes with "fit-for-purpose" samples to help you meet the needs of the laboratory.

Our proficiency testing (PT) program is a benchmark to:

  • provide staff with an insight into their laboratory′s performance, to verify the accuracy and reliability of their testing process
  • provide a comparison of performance with that of other laboratories, nationally and internationally
  • test and educate staff, provide a better understanding of the impact of incorrect results and identify areas where there may be problems
  • provide an independent evaluation of laboratory performance
  • demonstrate to clients, colleagues and accreditation bodies that there is a commitment to quality

Preparation Note


  • The effluent is provided as a concentrate for dilution in moderately hard synthetic freshwater (MHSF).
  • This concentrate is an EPA-specified toxicant in reagent-grade DI water.
  • Enough material has been provided to run the method at least twice. If you require more toxicant, please contact
  • Do not deviate from the supplied instructions or EPA Method 2000.0. Contact before running the test if you see the need to deviate from the test conditions. Deviations may affect your final evaluation.

Preparation Instructions

  • To prepare 1 L of effluent, add 900 mL of MHSF to a volumetric flask. Preparation instructions for MHSF can be found in the EPA document EPA-821-R-02-012, page 32.
  • Transfer the entire contents of one ampule to the flask. Rinse the ampule with MHSF and transfer to the volumetric flask. Bring flask to 1 L volume with MHSF.
  • The effluent prepared according to these instructions represents the sample ready for testing, 100% effluent. This 100 % effluent is split into two 0.5 L aliquots. The first aliquot is your 100% effluent for testing. The second aliquot is diluted with 0.5 L MHSF and mixed. This becomes your 50% effluent sample. Continue diluting half of each sample with the same volume of MHSF to make your 25%, 12.5% and 6.25% effluent dilutions, which represent the five test dilutions.

Required Test Conditions
  • Test Type: Static non-renewal
  • Test duration: 48 h,Temperature: 25 ºC ±1 ºC. The test must not deviate by more than 3 ºC during the test.
  • Renewal of Test solutions: non-renewal
  • Dilution water: Moderately hard synthetic water.

Test Conditions
  • Light quality: Ambient laboratory illumination (recommended)
  • Light intensity: 10-20 μE/m2/s (50-100 ft-c) (ambient laboratory levels) (recommended)
  • Photoperiod: 16 h light, 8 h darkness (recommended)
  • Test chamber size: 250 mL (recommended minimum)
  • Test solution volume: 200 mL (recommended minimum)
  • Age of test organisms: 1-14 days; less than or equal to 24-h range in age (required),
  • No. organisms per test chamber: 10 (required minimum)
  • No. replicate chambers per concentration: 2 (required minimum)
  • No. of organisms per concentration: 20 (required minimum)
  • Feeding regime: Artemia nauplii are made available while holding prior to the test
  • Test chamber cleaning: Cleaning not required
  • Test solution aeration: None, unless DO concentration falls below 4.0 mg/L; rate should not exceed 100 bubbles/min.
  • Test concentrations: 5 and a control (required minimum)
  • Dilution series: greater than or equal to 0.5 dilutions series (recommended)
  • Test acceptability criterion: 90% or greater survival in controls
  • Sample holding requirements: Composite sample within 36 h of effluent preparation (required)

Storage and Stability

Please refer to sample label for expiration date, specific storage temperature, and specific storage conditions.

Other Notes

Are you new to Supelco® Proficiency TestingͰ Be sure to register your account details in the PT Portal before placing your order.
This will provide access to data entry, reporting packets and final reports. Registrations will be approved shortly after your order is placed. For further questions, write to us at

When placing your order online, please indicate your study preference under the ′your reference′ column. Available studies can be viewed at the bottom of the page.
Click here to learn more about Quick-Turn Studies (on-demand proficiency tests that are offered depending on lot availability). The Quick-Turn results are released within 48-72 hours whereas scheduled study reports are released within 21 days.

Proficiency testing samples are presented as single blind. No values for the samples are released until the testing period concludes.

Whole Effluent Toxicity (WET) tests are designed to predict the impact and toxicity of effluents discharges

Proficiency Testing Schedule 2023

DMR-QA (US customers only)
DMR-QA 43 2023 Dates TBD

Quick-Turn Study (on demand proficiency test that is offereddepending on lot availability)

Legal Information

Supelco is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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