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Mysid (Mysidopsis bahia, Americamysis bahia), 7-day Chronic, daily renewal, SSW

Proficiency Testing Material


Quality Level


ampule of 14 × 2 mL


in water




matrix material

General description

This Whole Effluent Toxicity (WET) proficiency testing sample is produced in accordance with ISO/IEC 17043:2010. This sample is manufactured according to TNI requirements. However, please note, this sample may include non-TNI analytes as well.

In chronic WET tests, the shrimp-like crustacean, Mysid, is employed as a test organism to measure its ability to grow, develop and reproduce when exposed to wastewater effluents. This method of determination is used to estimate the effluent′s toxicity levels in aquatic/freshwater environments.

Supelco® is a global proficiency testing (PT) provider that manufactures best-in-class environmental and pharmaceutical PT products. Most water PT samples are available as concentrates or ready-to-use volumes giving the end-user flexibility in selecting samples that will fit their exact needs. Our PT program is designed to meet the quality assurance requirements in compliance with Discharge Monitoring Report - Quality Assurance Program (DMR-QA), thereby ensuring the analytical competence of your laboratory.

Analyte/s: Values of analytes vary lot to lot.

All information regarding the use of Mysid (Mysidopsis bahia, Americamysis bahia), 7-day Chronic, daily renewal, SSW proficiency testing standard can be found in the reporting packet which will be available to download at PT Portal.


Mysid (Mysidopsis bahia, Americamysis bahia), 7-day Chronic, daily renewal, SSW proficiency testing material is designed for use in Chronic Whole Effluent Toxicity (WET) proficiency testing.

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For more information on this product, please contact our PT Service Team.

Features and Benefits

We have a vast portfolio of environmental PT schemes with "fit-for-purpose" samples to help you meet the needs of the laboratory.

Our proficiency testing (PT) program is a benchmark to:

  • provide staff with an insight into their laboratory′s performance, to verify the accuracy and reliability of their testing process
  • provide a comparison of performance with that of other laboratories, nationally and internationally
  • test and educate staff, provide a better understanding of the impact of incorrect results and identify areas where there may be problems
  • provide an independent evaluation of laboratory performance
  • demonstrate to clients, colleagues and accreditation bodies that there is a commitment to quality

Preparation Note


  • The effluent is provided as a concentrate for dilution in synthetic seawater.
  • This concentrate is an EPA-specified toxicant in reagent-grade DI water.
  • Enough material has been provided to run the method at least twice. If you require more toxicant please contact An ampule is provided for making fresh effluents for each day of the test.
  • Do not deviate from the supplied instructions or EPA Method 1007.0. Contact before running the test if you see the need to deviate from the test conditions.
  • Deviations may affect your final evaluation

Preparation Instructions

  • To prepare 3 L of effluent, add 2900 mL of synthetic seawater to a volumetric flask.
  • Preparation instructions for synthetic seawater can be found in the EPA document EPA-821-R-02-014, page 26.
  • Transfer the entire contents of one ampule to the flask.
  • Rinse the ampule with synthetic seawater and transfer to the volumetric flask. Bring flask to 3 L volume with synthetic seawater.
  • This represents 3 L of effluent water for the test.

Required Test Conditions

  • Test Type: Static renewal
  • Test duration: 7 days
  • Temperature: 25 ºC ±1 ºC. The test must not deviate by more than 3 ºC during the test.
  • Renewal of Test solutions: Daily
  • Dilution water: Synthetic seawater


  • Light quality: Ambient laboratory illumination (recommended)
  • Light intensity: 10-20 μE/m2/s (50-100 ft-c.) (ambient laboratory levels) (recommended)
  • Photoperiod: 16 h light, 8 h darkness, with phase in/out period (recommended)
  • Test chamber: 8 oz plastic disposable cups, or 400 mL glass beakers (recommended)
  • Test solution volume: 150 mL per replicate (recommended minimum)
  • Age of test organisms: 7 days (required)
  • No. of organisms per test chamber: 5 (required minimum)
  • No. of replicate chambers per concentration: 8 (required minimum)
  • No. of larvae per concentration: 40 (required minimum)
  • Source of food: Newly hatched Artemia nauplii (less than 24 h old) (required)
  • Feeding regime: Feed 150 24 h old nauplii per mysid daily, half after test solution renewal and half after 8-12 h (recommended).
  • Pipette excess food from cups daily immediately before test solution renewal and feeding (recommended).
  • Aeration: None unless DO falls below 4.0 mg/L, then gently aerate in all cups (recommended).
  • Endpoints: Survival and growth (required); and egg development (recommended)
  • Test acceptability criteria: 80% or greater survival, average dry weight 0.20 mg or greater in controls (required); fecundity may be used if 50% or more of females in controls produce eggs (required if fecundity endpoint used).

Storage and Stability

  • Please refer to sample label for expiration date, specific storage temperature, and specific storage conditions.
  • If storage conditions are not provided on the label, it is intended for room temperature storage.

Other Notes

Are you new to Supelco® Proficiency TestingͰ Be sure to register your account details in the PT Portal before placing your order.
This will provide access to data entry, reporting packets and final reports. Registrations will be approved shortly after your order is placed. For further questions, write to us at

When placing your order online, please indicate your study preference under the ′your reference′ column. Available studies can be viewed at the bottom of the page.
Click here to learn more about Quick-Turn Studies (on-demand proficiency tests that are offered depending on lot availability). The Quick-Turn results are released within 48-72 hours whereas scheduled study reports are released within 21 days.

Proficiency testing samples are presented as single blind. No values for the samples are released until the testing period concludes.

Whole Effluent Toxicity (WET) tests are designed to predict the impact and toxicity of effluents discharges.

Proficiency Testing Schedule 2023

DMR-QA (US customers only)
DMR-QA 43 2023 Dates TBD

Quick-Turn Study (on demand proficiency test that is offereddepending on lot availability)

Legal Information

Supelco is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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