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Indo-1FF-AM

Name: Indo-1FF-AM
Brand: SIGMA

suitable for fluorescence, ≥95% (TLC)

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Empirical Formula (Hill Notation):

C₄₆H₄₇F₂N₃O₂₂

Molecular Weight:

1031.87

MDL number:

MFCD01867225

PubChem Substance ID:

57647603

Quality Level

100

Assay

≥95% (TLC)

solubility

DMSO: soluble

fluorescence

λ_ex 350 nm; λ_em 435 nm in 0.1 M Tris pH 8.0, esterase; 10 mM Ca²⁺

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccc(cc1OCCOc2c(F)c(F)ccc2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)-c3c[nH]c4cc(ccc34)C(=O)OCOC(C)=O

InChI

1S/C46H47F2N3O22/c1-26(52)64-21-69-40(57)17-50(18-41(58)70-22-65-27(2)53)37-10-7-31(34-16-49-36-14-32(6-8-33(34)36)46(61)73-25-68-30(5)56)15-39(37)62-12-13-63-45-38(11-9-35(47)44(45)48)51(19-42(59)71-23-66-28(3)54)20-43(60)72-24-67-29(4)55/h6-11,14-16,49H,12-13,17-25H2,1-5H3

InChI key

VSJCENCIDAGWSX-UHFFFAOYSA-N

Features and Benefits

Pale yellow green solid. Cell permeable acetoxymethyl ester (AM) derivative of Indo-1FF with spectral properties similar to the parent compound. A ratiometric dye with the same fluorescent properties and similar applications as Indo-1, but has a lower affinity for calcium, and is insensitive to magnesium

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Use of Indo-1FF for measurements of rapid micromolar cytoplasmic free Ca2+ increments in a single smooth muscle cell.

Ganitkevich VYa

Cell calcium, 23(5), 313-322 (1998-07-29)

A low-affinity fluorescent Ca2+ indicator Indo-1FF was used to measure cytoplasmic Ca2+ increments in single smooth muscle cells isolated from the urinary bladder of the guinea-pig. The in vitro Kd of Indo-1FF for Ca2+ measured at the microscope stage was

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