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Merck
CN

B5525

Bromophenol Blue sodium salt

Synonym(s):

3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt

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About This Item

Empirical Formula (Hill Notation):
C19H9Br4NaO5S
CAS Number:
34725-61-6
Molecular Weight:
691.94
UNSPSC Code:
12171500
NACRES:
NA.52
PubChem Substance ID:
57653941
EC Number:
252-170-2
Beilstein/REAXYS Number:
6995701
MDL number:
MFCD00013793

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InChI key

TWKDJMRHSLWRFI-UHFFFAOYSA-M

InChI

1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1

SMILES string

[Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O

form

powder

technique(s)

electrophoresis: suitable

solubility

water: soluble

suitability

suitable for nucleic acid electrophoresis

storage temp.

room temp

Quality Level

200

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Application

A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.
Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis. It has also been used in Drosphila fly food to aid visualization of gut for dissection.

General description

Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Preparation Note

A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT2664
0000483860
0000483860
0000483663
0000483663

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