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B5525

Bromophenol Blue sodium salt

Name: Bromophenol Blue sodium salt
Brand: SIGMA

Synonym(s):

3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt

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About This Item

Empirical Formula (Hill Notation):

C₁₉H₉Br₄NaO₅S

CAS Number:

34725-61-6

Molecular Weight:

691.94

UNSPSC Code:

12171500

NACRES:

NA.52

PubChem Substance ID:

57653941

EC Number:

252-170-2

Beilstein/REAXYS Number:

6995701

MDL number:

MFCD00013793

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form

powder

Quality Level

200

technique(s)

electrophoresis: suitable

solubility

water: soluble

suitability

suitable for nucleic acid electrophoresis

storage temp.

room temp

SMILES string

[Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O

InChI

1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1

InChI key

TWKDJMRHSLWRFI-UHFFFAOYSA-M

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Clinical & Diagnostics

Dry Dyes & Certified Stains

Tissue Diagnostics

General description

Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis. It has also been used in Drosphila fly food to aid visualization of gut for dissection.

Preparation Note

A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

SHBT2664

0000483860

0000483663

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Global Trade Item Number

SKU	GTIN
B5525-10G	04061835572076
B5525-5G	04061835572090
B5525-25G	04061835572083

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