Skip to Content
Merck
CN

B5655

Sigma-Aldrich

BCIP®/NBT

SIGMAFAST™, alkaline phosphatase substrate, chromogenic, tablet

Synonym(s):

BCIP/NBT Substrate, BCIP®/NBT Alkaline Phosphatase Substrate

Sign Into View Organizational & Contract Pricing

Select a Size

About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

SIGMAFAST™ BCIP®/NBT, tablet

form

tablet

solubility

water: 10 mL, clear, yellow

storage temp.

−20°C

Related Categories

Application

SIGMAFAST BCIP®/NBT has been used as a substrate
  • for the anti-mouse IgG conjugated alkaline phosphatase in immunoblotting
  • for alkaline phosphatase in the histochemical staining of primary osteoblasts
  • for alkaline phosphatase staining in bone marrow stromal cells

Preparation Note

Each tablet dissolved in 10 ml deionized water yields a ready-to-use buffered solution containing BCIP/NBT, pH 9.5.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000468886
0000468886
0000442829
0000442829
0000411613

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Mitogen-activated protein kinase phosphatase 1 regulates bone mass, osteoblast gene expression, and responsiveness to parathyroid hormone
Mahalingam CD, et al.
The Journal of Endocrinology, 211(2), 145-156 (2011)
Nicolas Leventoux et al.
Stem cell research, 47, 101896-101896 (2020-07-14)
Amyotrophic Lateral Sclerosis is the most common motor neuron degenerative disease in adults, and TARDBP gene mutations have been reported to be involved in the pathogenesis. We present here how we generated the human induced pluripotent stem cell (hiPSC) line
MicroRNA-183-5p increases with age in bone-derived extracellular vesicles, suppresses bone marrow stromal (stem) cell proliferation, and induces stem cell senescence
Davis C, et al.
Tissue Engineering: Part A, 23(21-22), 1231-1240 (2017)
Sialic acid on herpes simplex virus type 1 envelope glycoproteins is required for efficient infection of cells
Teuton JR and Brandt CR
Journal of Virology, 81(8), 3731-3739 (2007)
R Gonzalez-Castro et al.
Inflammopharmacology, 26(3), 817-827 (2017-11-03)
The main amyloid-beta (Aβ) variants detected in the human brain are full-length Aβ1-40 and Aβ1-42 peptides; however, a significant proportion of AD brain Aβ consists also of N-terminal truncated/modified species. The majority of the previous immunotherapeutic strategies targeted the N-terminal
View All Related Papers

Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

Protocols

Immunodetection Using BCIP/NBT Substrate

BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Guide: Expansion & Differentiation of Human Mesenchymal

TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.

免疫组织化学操作步骤

本实验方案通过对石蜡包埋的组织切片中的特定抗原进行染色和成像，完成整个免疫组织化学（IHC）操作步骤。

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service