CMC0001
SIG10 Chemically Competent Cells
for protein expression and DNA plasmid production
biological source
Escherichia coli
grade
for molecular biology
form
buffered aqueous solution
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: chemically competent
transformation efficiency: ≥1 × 109 cfu/μg
shipped in
dry ice
storage temp.
−70°C
Related Categories
General description
These cells are ideal for cloning and propagation of plasmid, cosmid, or fosmid clones and are highly efficient for routine cloning applications.
They share the most useful genetic elements of standard cloning strains like DH5α™ DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols.
They are are provided in 40 μL, 80 μL and 160 μL aliquots, each being sufficient for one, two and four transformations respectively.
The cells have a transformation efficiency of >1 x 109 cfu/μg
The 96-well format are provided in aliquots of 20 μL per well and have a transformation efficiency of >1 x 108 cfu/μg.
Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA
Application
Features and Benefits
- share the most useful genetic elements of standard cloning strains like DH5α™ DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols.
- ensures recovery of stable and high quality plasmid DNA.
- high transformation efficiency
- provide the performance researchers need with ease of use.
- provide solutions for a wide range of applications at economical prices.
- Blue - white screening
Components
- SIG10 chemically competent cells
- pUC 19 transformation control DNA at 10 pg/μL
- recovery medium for cloning
Principle
This strain also carries the recA1 and endA1 mutations. The recA1 genotype provides minimized recombination and aids in plasmid stability while endA1 provides for high quality plasmid DNA preparation.
They are bacteriophage T1-resistant (tonA mutation) and also resistant to streptomycin by virtue of rpsL mutation.
Legal Information
related product
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.
Difficulty Finding Your Product Or Lot/Batch Number?
How to Find the Product Number
Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).
Example:
Additional examples:
705578-5MG-PW
PL860-CGA/SHF-1EA
MMYOMAG-74K-13
1000309185
enter as 1.000309185)
Having trouble? Feel free to contact Technical Service for assistance.
How to Find a Lot/Batch Number for COA
Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.
Aldrich Products
For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').
For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').
For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.
Not Finding What You Are Looking For?
In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.
Articles
Colony PCR reagents and our colony PCR protocol using REDExtract-N-Amp™ PCR ReadyMix™ and JumpStart™ REDTaq® PCR ReadyMix™ reagents.
Protocols
For best results, ligation reactions must be heat inactivated at 70º C for 15 minutes before transformation. Alternately, the reactions may be purified.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service