G0799
β-Glucuronidase from Escherichia coli
>20,000,000 units/g protein, recombinant, expressed in E. coli, aqueous glycerol solution
Synonym(s):
β-D-Glucuronide glucuronosohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
Specific activity:
>20,000,000 units/g protein
Recombinant:
expressed in E. coli
Concentration:
>1 mg/mL
recombinant
expressed in E. coli
Quality Level
form
aqueous glycerol solution
specific activity
>20,000,000 units/g protein
mol wt
74 kDa
concentration
>1 mg/mL
shipped in
wet ice
storage temp.
−20°C
General description
β-Glucuronidase is a tetramer belonging to the glycosidase family with a molecular weight of 29 kDa. It is composed of 74 kDa identical monomers. The pI and optimal pH of the enzyme is 4.8 and 6-7 respectively.
Application
β-Glucuronidase was used in the study of Cer-β-glucuronide synthesis and evaluation of β-glucuronidase(s) from Escherichia coli or intestinal segments to release the head group.
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Effective in the hydrolysis of steroid glucuronides.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Physical form
β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.
Highly purified solution in 50% glycerol
Other Notes
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).
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Danger
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
wgk
WGK 1
Regulatory Information
常规特殊物品
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Protocols
Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.
为了使用β-葡糖醛酸酶优化水解，必须对每种待分析的葡糖苷酸代谢物进行某些因素的评估，例如孵育时间、温度、水解pH、酶源和酶浓度。
D H Kim et al.
Biological & pharmaceutical bulletin, 18(9), 1184-1188 (1995-09-01)
beta-Glucuronidase was purified 360-fold from Escherichia coli HGU-3, an human intestinal bacterium. The specific activity of the purified enzyme was 17.78 units/mg protein. The enzyme (M.W. 290000) is composed of four subunits (M.W. 72000) with a pI and optimal pH
E M Schmelz et al.
Cancer research, 59(22), 5768-5772 (1999-12-03)
Dietary sphingolipids inhibit chemically induced colon cancer in mice. The most likely mediators of this effect are the metabolites ceramide (Cer) and sphingosine, which induce growth arrest and apoptosis in transformed cells. Sphingolipids are digested in both the upper and
Amichay Meirovitz et al.
The FEBS journal, 280(10), 2307-2319 (2013-02-13)
Recent years have seen a growing body of evidence that enzymatic remodeling of heparan sulfate proteoglycans profoundly affects a variety of physiological and pathological processes, including inflammation, neovascularization, and tumor development. Heparanase is the sole mammalian endoglycosidase that cleaves heparan
Global Trade Item Number
| SKU | GTIN |
|---|---|
| G0799-100KU | 04061832843650 |
| G0799-25KU | 04061832843667 |