HSRT100
Enhanced Avian HS RT-PCR Kit
Flexible kit for one-step or two-step RT-PCR
Select a Size
About This Item
Quality Level
usage
sufficient for 100 reactions
feature
dNTPs included
hotstart
technique(s)
RT-PCR: suitable
color
colorless
input
purified RNA
shipped in
wet ice
storage temp.
−20°C
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General description
One-step: In a single tube, eAMV™ RT and AccuTaq LA act sequentially to first produce cDNA and then immediately amplify by PCR. This provides quick, sensitive analysis of RNA.
Two-step: Each reaction is individually optimized for greater yields with high fidelity, when protocol requires multiple amplifications, or if maximum yield is more important than maximum convenience.
Application
Features and Benefits
- Greater transcription lengths than other reverse transcriptases, generating cDNA up to 14.1 Kb.
- Higher sensitivity for detecting low abundance messages. eAMV™ RT is able to transcribe RNA that other reverse transcriptases cannot detect.
- Unsurpassed transcription through difficult secondary structure.
- Increased sensitivity, specificity and yield from JumpStart AccuTaq LA DNA Polymerase.
Other Notes
Legal Information
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Articles
Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.
分析基因表达的方法之一是测量基因的mRNA浓度。此类分析面临着若干挑战，例如不同转录本之间的半衰期不同、转录时间模式以及mRNA和蛋白质之间缺乏相关性。
Protocols
REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.
The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.
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