I9516
2-Propanol
for molecular biology, BioReagent, ≥99.5%
grade
for molecular biology
Quality Level
vapor density
2.1 (vs air)
vapor pressure
33 mmHg ( 20 °C)
44 mmHg ( 25 °C)
product line
BioReagent
Assay
≥99.5%
form
liquid
autoignition temp.
750 °F
expl. lim.
2.0-12.7 %, 93 °C
technique(s)
DNA extraction: suitable
RNA extraction: suitable
impurities
≤0.2% water
water
color
colorless
refractive index
n20/D 1.377 (lit.)
bp
82 °C (lit.)
mp
−89.5 °C (lit.)
solubility
water: soluble (completely)
density
0.785 g/mL at 25 °C (lit.)
suitability
suitable for precipitation of nucleic acids
storage temp.
room temp
SMILES string
CC(C)O
InChI
1S/C3H8O/c1-3(2)4/h3-4H,1-2H3
InChI key
KFZMGEQAYNKOFK-UHFFFAOYSA-N
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General description
Protocols for the use of isopropanol:
- DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues.
- Protein crystallization.
- Isolate retinoids from biological matrices for HPLC and HPLC-MS analysis.
Application
- in DNA and RNA extraction
- for determination of hydroxyproline levels(The Merck Index, 12th ed)
- for checking cell viability by MTT assay
- in microarray hybridization
also commonly purchased with this product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
Target Organs
Central nervous system
Storage Class Code
3 - Flammable liquids
WGK
WGK 1
Flash Point(F)
53.6 °F - closed cup
Flash Point(C)
12.0 °C - closed cup
Personal Protective Equipment
Regulatory Information
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Protocols
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Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.
Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents
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