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L0418

Sigma-Aldrich

LB Agar Carbenicillin - 100, Plates

pre-poured agar plates with 100 μg/ml carbenicillin

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Synonym(s):
carbenicillin agar plates

grade

for molecular biology

sterility

sterile; autoclaved

form

plates (10 cm)
plates (10cm, 20-25ml each)

availability

available only in USA

capacity

20-25 mL

storage temp.

2-8°C

suitability

selective for Escherichia coli
selective for coliforms

General description

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

LB Agar Carbenicillin - 100, Plates may be used to culture transformed bacteria with an antibiotic-resistant plasmid using a microwave oven.
Suitable for selective cultivation of E. coli strains that contain plasmids conferring carbenicillin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins.

Features and Benefits

Miller′s LB Agar plates provide:
  • Convenient, ready-to-use 10cm format
  • Standard formulation plus antibiotic

Components

15 g/L Agar
10 g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract
100 μg/ml carbenicillin

Other Notes

The plates must be used within two months of the manufacture date to ensure the antibiotics are fully active. The product may be shipped up until its expiration date but is generally shipped 2-4 weeks before expiration.

related product

Product No.
Description
Pricing

C3416

Carbenicillin disodium salt, BioReagent, suitable for plant cell culture

L3147

LB Broth with agar (Miller), Highly-referenced nutrient-rich microbial growth powder medium with Agar, suitable for regular E.coli culture.

L5542

LB Agar Plates, pre-poured LB agar plates for E. coli

L5667

LB Agar Ampicillin-100, Plates, pre-poured LB agar plates with 100 μg/ml ampicillin

L0168

LB Agar Ampicillin - 50, Plates, pre-poured LB agar plates with 50 μg/ml ampicillin

CMC0001

SIG10 Chemically Competent Cells, for protein expression and DNA plasmid production

CMC0007

SIG10 5a Chemically Competent Cells, for DNA plasmid production

CMC0014

BL21(DE3) Chemically Competent Cells, for protein expression

CMC0017

OverExpress™ C41(DE3) Chemically Competent Cells, for the highest protein expression

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物来源培养基

Certificates of Analysis (COA)

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T1503
Product Number
-
25G
Pack Size/Quantity

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Why do the LB-agar plates with antibiotics have such a short expiration date?

    The antibiotics in the LB-agar plates are not stable.  The antibiotic degrades over time, making it ineffective at selecting transformed bacteria grown on the plate.  Because of this, we recommend the plates are used as soon as possible once they are received.  For labs with low use, the plates may expire quickly, so they may benefit from pouring their own plates using the LB - Miller powder with agar formulation (L3147).

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Sterile preparation of antibiotic-selective LB agar plates using a microwave oven
Iacoviello Michael P and Rubin SA
Biotechniques, 30(5), 963-965 (2001)

Articles

Protein Expression Systems

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.

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