L2020
Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane
1-2 mg/mL in Tris-buffered saline, 0.2 μm filtered, BioReagent, suitable for cell culture
biological source
mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)
Quality Level
product line
BioReagent
form
aqueous solution
mol wt
A subunit 400 kDa
B1 subunit 210 kDa
B2 subunit 200 kDa
packaging
pkg of 1 mg
concentration
1-2 mg/mL in Tris-buffered saline
technique(s)
cell culture | mammalian: suitable
surface coverage
1‑2 μg/cm2
impurities
Microbial Contamination, passes test
NCBI accession no.
Binding Specificity
Peptide Source: Collagen
shipped in
dry ice
storage temp.
−20°C
Gene Information
mouse ... Lama1(16772) , Lamb1(16777) , Lamc1(226519) , Lamc2(16782)
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Related Categories
General description
Application
- insulin-producing cell (IPC) differentiation.
- Infection inhibition assays.
- Proliferation Assay.
Biochem/physiol Actions
Components
Caution
Preparation Note
related product
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Certificates of Analysis (COA)
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For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
What is the protein concentration of the solution of Product L2020, Laminin?
The protein specification for L2020 is 1.0 to 2.0 mg/mL. The exact value can be found on the lot-specific Certificate of Analysis, which can be downloaded from our website.
What type of laminin is Product L2020?
In the nomenclature system by Burgeson, this is Laminin 1. The laminins were numbered with Arabic numerals in the order discovered. The previous A, B1, and B2 chains, and their isoforms are listed by the Greek letters, for alpha, beta, and gamma, respectively, followed by an Arabic numeral to identify the isoforms. For example, the first laminin identified from the Engelbreth-Holm-Swarm tumor (EHS) was designated laminin-1 with the chain composition α-1/β-1/γ-1. The genes for these 3 chains are LAMA1, LAMB1, and LAMC1. Reference: Burgeson, R.E., et al., A new nomenclature for the laminins. Matrix Biol., 14, 209-211 (1994).
What is the purity of Laminin, Product L2020?
We do not determine a purity of this product. An SDS PAGE gel is run and the pattern (3 bands) is comparable to past lots.
Can I coat Laminin, Product L2020, on top of poly-L-lysine for better adherence?
When coating coverslips or slides for use with neural stem cells (neurospheres), the following procedure is recommended. Precoat the coverslips with poly-L-lysine (Product No. P5899) at 100 μg/mL. This is incubated for 30 minutes or more at room temperature. The coverslips are then washed with sterile water and allowed to dry. The poly-lysine coated coverslips can be stored at room temperature until ready to use (up to ~1 month). Coverslips are then coated with 30 - 50 μL of warmed laminin, which is at a concentration of 50 μg/mL. Incubate at 37°C for 30 minutes. Remove the laminin, and wash with DME (50 μL) twice. Cells are then added to the coverslips.
How can I store solutions of Laminin, Product L2020?
The product has a recommended storage temperature of -20°C. Please note, the product needs to be thawed slowly at 2-8°C. If the product has been warmed up at room temperature and it gels, it cannot be reactivated for use. We recommend storage at 2-8°C for up to one week. For longer storage, the product needs to be frozen at -20°C in single-use aliquots.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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