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Merck
CN

NA2110

Sigma-Aldrich

GenElute™ Bacterial Genomic DNA Kits

sufficient for 70 purifications

Synonym(s):

Bacterial Genomic DNA, bacterial DNA prep, bacterial DNA purification kit, bacterial gDNA extraction kit, bacterial gDNA isolation kit, bacterial gDNA purification kit, bacterial genomic DNA extraction kit, bacterial genomic DNA isolation kit, bacterial genomic DNA purification kit, Gen Elute

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About This Item

UNSPSC Code:
41105501
NACRES:
NA.55

Key Documents

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usage

sufficient for 70 purifications

Quality Level

200

technique(s)

DNA purification: suitable

storage temp.

15-25°C

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General description

Click here to view the kit protocol
The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.

The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Application

The purified bacterial genomic DNA is ready for downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • cloning

Biochem/physiol Actions

The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution.

The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Features and Benefits

  • Starting material: Up to 1.5 mL of culture
  • Expected yield: Up to 20 μg
  • Elution volume: 400 μl
  • Time required: 70 - 120 min
  • A260/A280 ratio: 1.6 - 1.9
  • No phenol, chloroform, or ethanol precipitation required
  • Typical DNA yields of 15 μg - 20 μg
  • Protocols provided for Gram + and Gram - bacteria
  • High quality genomic DNA in less than 2 hours
  • Purified DNA has an A260/A280 ratio between 1.6 and 1.9

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS
  • P2308Proteinase K from Tritirachium album, lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg protein 3SDS
  • C2112Column Preparation SolutionSDS
  • R6148RNase A solutionSDS
  • W0263Wash Solution 1SDS

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system, Respiratory system

Storage Class Code

3 - Flammable liquids

Flash Point(F)

77.0 °F - closed cup

Flash Point(C)

25 °C - closed cup

Regulatory Information

常规特殊物品
危险化学品
低风险生物材料
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Certificates of Analysis (COA)

Lot/Batch Number
0000484281
0000484281
0000469633
0000469633
0000377133

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