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P2443

Sigma-Aldrich

Pluronic® F-127

powder, BioReagent, suitable for cell culture

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Synonym(s):
Non-ionic copolymer surfactant
Linear Formula:
(C3H6O·C2H4O)x
CAS Number:
9003-11-6
MDL number:
MFCD00082049
PubChem Substance ID:
24897874
NACRES:
NA.75

description

Non-ionic
contains 100ppm BHT

Quality Level

200

product line

BioReagent

form

powder

mol wt

~12600 g/mol

technique(s)

cell culture | mammalian: suitable

CMC

950-1000 ppm (~25°C)

transition temp

cloud point >100 °C

HLB

18 - 23

InChI

1S/C3H6O.C2H4O/c1-3-2-4-3;1-2-3-1/h3H,2H2,1H3;1-2H2

InChI key

RVGRUAULSDPKGF-UHFFFAOYSA-N

Related Categories

General description

Pluronicpluronic® F-127, also known as poloxamer 407, is a non-toxic and nonionic copolymer. It contains polyethylene oxide-polypropylene oxide arranged in a triblock structure to form PEO-PPO-PEO symmetric. Surfactant properties of this polymer derived from its amphiphilic nature, allow them to interact with hydrophobic surfaces and biological membranes.

Application

Pluronic® F-127 has been used:

  • as a delivery system for a peptide to the wound site to study the effect of the peptide on wound healing in mice.
  • as a surface coating for PDMS (polydimethylsiloxane) stamps to make them hydrogel repellent.
  • in the preparation of calcium indicator stock solution for measuring neural activities.
  • as a component of phosphate-buffered saline (PBS), to lower unspecific cell and protein adhesion to a PDMS-based microfluidic device.
  • This non-ionic copolymer surfactant is suitable for use in insect cell culture applications as an antifoaming agent.

Biochem/physiol Actions

Pluronic® F-127 is a thermo-reversible gel that finds its application in different industries due to its high thermosensitivity, biodegradability, and biocompatibility properties. These properties make this polymer suitable for use as a versatile drug carrier.Pluronic® F-127 improves the therapeutic efficiency of drugs by increasing their bioavailability and thermostability. Additionally, it can promote cellular attachment and collagen formation, which facilitates angiogenesis and tissue regeneration. Due to its efficacy, Pluronic F-127 has been utilized in the encapsulation of mesenchymal stem cells (MSCs) to aid in the regeneration of tissues with low levels of vascularity, such as epithelial tissues, adipose tissues, cartilage, tendons, and bone tissues.

Legal Information

Pluronic is a registered trademark of BASF

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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25G
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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the molecular weight of Pluronic F-127, Product P2443?

    The average molecular weight is 12.6 kDa.

  4. Why has my solution of Pluronic F-127, Product P2443, gelled?

    Pluronic F-127 will form gels above 10 °C when used at concentrations of 18-50%. It will re-liquefy when cooled to below 10 °C. The gel should remain stable during autoclaving.

  5. What concentration of Pluronic F-127, Product P2443, should be used in cell culture?

    We test Pluronic F-127 in cell culture at 0.1 g/100 ml media (0.1%).  The concentration necessary for your cell application may vary.

  6. What can I use to dilute Pluronic F-127, Product P2443?

    The Pluronic F-127 is water soluble as stated on the Specification Sheet.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

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Environmentally responsive injectable materials
Bearat HH and Vernon BL
Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine, 263-297 (2011)
Dual-responsive (pH/temperature) Pluronic F-127 hydrogel drug delivery system for textile-based transdermal therapy
Chatterjee S, et al.
Scientific Reports, 9(1) (2019)
Connexin43 carboxyl-terminal peptides reduce scar progenitor and promote regenerative healing following skin wounding
Ghatnekar GS, et al.
Regenerative Medicine, 4(2), 205-223 (2009)
PLURONIC F127 and ITS applications
Salama AH
Pharmacology, 2, 1393- 1403 (2021)
Philippe Marmottant et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(41), 17271-17275 (2009-10-07)
Cell aggregates are a tool for in vitro studies of morphogenesis, cancer invasion, and tissue engineering. They respond to mechanical forces as a complex rather than simple liquid. To change an aggregate's shape, cells have to overcome energy barriers. If
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