P4557
Phenol solution
Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology
grade
for molecular biology
Quality Level
product line
BioReagent
Assay
≥99.0% (GC)
form
liquid
pH
7.7-8.1 (25 °C)
storage temp.
2-8°C
SMILES string
Oc1ccccc1
InChI
1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H
InChI key
ISWSIDIOOBJBQZ-UHFFFAOYSA-N
Gene Information
human ... GABRA1(2554)
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General description
Application
- as a component of the digestion reaction mix to prepare RNase-free plasmids for in vitro translation in a rabbit reticulocyte lysate
- for extraction of DNA from Arabidopsis thaliana Rosette leaves
- in the extraction of DNA from blood on preservation paper by standard phenol-chloroform protocol
- as a component of extraction buffer to extract RNA from fruit pulp
- for the extraction of protein from roots
- for the isolation of fecal DNA
- for the extraction of caged RNA
Biochem/physiol Actions
Components
- 1 bottle Tris-equilabrated Phenol (product number P4557)
- Equilibration Buffer (product B5658)
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
Storage Class Code
6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Example:
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705578-5MG-PW
PL860-CGA/SHF-1EA
MMYOMAG-74K-13
1000309185
enter as 1.000309185)
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Aldrich Products
For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').
For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the molarity of Phenol solution, Product P4557?
The Molarity is ~10.6. The density of the phenol is 1.05g/ml, and the molecular weight is 94.11.
I see that there are two layers in the bottle Phenol solution, Product P4557. Which layer do I use?
The top phase is Tris buffer, so use the lower/bottom phase.
What do I do to equilibrate the Phenol solution, Product P4557, to around pH 8?
This phenol solution (Product No. P4557) includes a separate bottle of equilibration buffer (Product No. B5658) to adjust the phenol phase to pH 7.9±0.2. After addition of the buffer to the phenol, it may take about 6 hours (mixture can be kept at 2-8 °C) until the bottom phase (phenol) becomes clear. Some of the haziness in the phenol phase may be due to some air bubbles caused by mixing by inversion. There could be a hazy interface between the phenol and aqueous phase which remains after the separation. The entire contents of the bottle of the buffer should be added to the phenol. If buffering less than the complete bottle, use at a ratio of 6.5 mL buffer to 100 mL phenol. For 10 mL of phenol, use 650 μL of buffer. For 1 mL of phenol, use 65 μL of buffer.
What is the final pH of Phenol solution, Product P4557, after adding the buffer?
The pH is typically 8.0; the specifications state a range of 7.7-8.1.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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