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P4557

Phenol solution

Name: Phenol solution
Brand: SIGMA

BioReagent, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, Molecular Biology

Synonym(s):

Tris-equilabrated Phenol

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About This Item

Empirical Formula (Hill Notation):

C₆H₆O

CAS Number:

108-95-2

Molecular Weight:

94.11

UNSPSC Code:

12352104

NACRES:

NA.52

PubChem Substance ID:

57654529

EC Number:

203-632-7

Beilstein/REAXYS Number:

6428463

MDL number:

MFCD00002143

Assay:

≥99.0% (GC)

Grade:

Molecular Biology

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grade

Molecular Biology

Quality Level

200

product line

BioReagent

assay

≥99.0% (GC)

form

liquid

dilution

(for analytical testing)

pH

7.7-8.1 (25 °C)

storage temp.

2-8°C

SMILES string

Oc1ccccc1

InChI

1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H

InChI key

ISWSIDIOOBJBQZ-UHFFFAOYSA-N

Gene Information

human ... GABRA1(2554)

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General description

A tris-equilibrated phenol solution is a clear liquid capped with a layer of Tris buffer. Phenol solutions can be used for a wide variety of molecular biology applications. Organic compounds like phenols have an aromatic carbocyclic ring with a hydroxyl group attached directly to it. The common name for monohydroxybenzene is phenol.

Application

Phenol solution has been used:

as a component of the digestion reaction mix to prepare RNase-free plasmids for in vitro translation in a rabbit reticulocyte lysate
for extraction of DNA from Arabidopsis thaliana Rosette leaves
in the extraction of DNA from blood on preservation paper by standard phenol-chloroform protocol
as a component of extraction buffer to extract RNA from fruit pulp
for the extraction of protein from roots
for the isolation of fecal DNA
for the extraction of caged RNA

Biochem/physiol Actions

The phenol-chloroform extraction method is widely employed to extract nucleic acids. Even though phenol does not completely inhibit RNase activity, its unique characteristic makes it beneficial for the isolation of nucleic acids when used in combination with chloroform and alcohol.

Other Notes

1 bottle Tris-equilabrated Phenol (product number P4557)
Equilibration Buffer (product B5658)

pictograms

GHS06,GHS08,GHS05,GHS09

signalword

Danger

hcodes

H301 + H311 + H331,H314,H341,H373,H411

pcodes

P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

target_organs

Nervous system,Kidney,Liver,Skin

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Regulatory Information

危险化学品

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Fecal Microbiome and Food Allergy in Pediatric Atopic Dermatitis: A Cross-Sectional Pilot Study

Fieten KB, et al.

International Archives of Allergy and Immunology, 175(1-2), 77-84 (2018)

Nucleic Acid Extraction Techniques

Yi-Wei Tang and Charles W. Stratton

Journal of Separation Science (2012)

Protocol Optimization of Proteomic Analysis of Korean Ginseng (Panax ginseng Meyer)

Braccia C, et al.

Journal of Separation Science, 8(4), 53-53 (2021)

PHENOLS

Smith PWG and Tatchell AR

Aromatic Chemistry, 79-104 (1969)

Detection and verification of Mycobacterium avium subsp. paratuberculosis in fresh ileocolonic mucosal biopsy specimens from individuals with and without Crohn's disease.

Tim J Bull et al.

Journal of clinical microbiology, 41(7), 2915-2923 (2003-07-05)

Mycobacterium avium subsp. paratuberculosis is a robust and phenotypically versatile pathogen which causes chronic inflammation of the intestine in many species, including primates. M. avium subsp. paratuberculosis infection is widespread in domestic livestock and is present in retail pasteurized cows'

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Global Trade Item Number

SKU	GTIN
P4557-100ML	04061837898792
P4557-400ML	04061837898808

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