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Sigma-Aldrich

Precast Agarose Gels

8-well (portrait orientation)

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Synonym(s):
8-well Precast Agarose Gels, Precast agarose gels for nucleic acid separation
NACRES:
NB.22

grade

for molecular biology

Quality Level

200

form

gel form

does not contain

ethidium bromide

concentration

1.25% agarose (in 1x MOPS buffer; for RNA electrophoresis)

storage temp.

room temp

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General description

Precast agarose gels are designed for high resolution separation of nucleic acids, producing sharp RNA bands with low background fluorescence. Precast gels are individually foil sealed in plastic trays and are supplied in a box of twenty.

Gel size: 6.0 × 9.5 cm, 5.5 mm thick
Cassette size: 6.8 × 10.2 cm container tray (fits common horizontal electrophoresis units)
Well orientation: 8-well, portrait style

Application

Precast Agarose Gels has been used for the electrophoresis of purified PCR products.
Precast agarose gels are designed for high resolution separation of nucleic acids, producing sharp DNA and RNA bands with low background fluorescence. Precast gels are individually foil sealed in plastic trays that help eliminate contamination from equipment and handling. Gels can be electrophoresed right in the plastic tray.

TBE - agarose gels are available in two common concentrations (1% and 4%) for DNA electrophoresis. Ethidium bromide (0.5 μg/ml) is included in the TBE-agarose gels for easy visualization. TBE-agarose gels are available in 8, 20 and 24 well formats. The separation range for the 1.0% gel is 500-1000 bp and 8-1000 bp for the 4% gel.

MOPS - agarose gels (1.25%) are available without denaturants (e.g. formaldehyde or glyoxal) for the analysis of total RNA, in vitro RNA transcripts, and Northern blotting. RNA electrophoresis usually does not interfere with staining. If significant secondary structure is suspected, the RNA should be denatured by a method listed in the product insert. The separation range for the 1.25% MOPS gel is 0.25-10 kb.

Packaging

Package of 20 individually foil-wrapped gels.

Other Notes

DNA agarose gels contain 1× TBE buffer (0.089 M Tris-borate, pH 8.3, 2 mM EDTA) and 0.5 mg/ml ethidium bromide.
RNA agarose gels contain 1× MOPS (20 mM MOPS, 5 mM sodium acetate, 1 mM EDTA and 1 mM EGTA, pH 7.0)

Quality

No detected DNase or RNase activity

Physical form

Gel size: 6.0 × 9.5 cm, 5.5 mm thick
Cassette size: 6.8 × 10.2 cm container tray; fits common horizontal electrophoresis units
Sample format: 8-well (portrait), 20-well (landscape), 24-well (2 × 12 well portrait)

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Nikolai A Shevchuk et al.
Nucleic acids research, 32(2), e19-e19 (2004-01-24)
A procedure for precise assembly of linear DNA constructs as long as 20 kb is proposed. The method, which we call long multiple fusion, has been used to assemble up to four fragments simultaneously (for a 10.8 kb final product)
Claudia S Meier-Trummer et al.
PloS one, 4(7), e6265-e6265 (2009-07-16)
Malignant catarrhal fever (MCF) is a lethal disease of cattle, characterized by vasculitis, necrosis, and accumulation of activated, dysregulated cytotoxic lymphocytes in various tissues. Ovine gamma herpesvirus 2 (OvHV-2) is a causative agent of MCF, which may trigger the disease

Protocols

Precast Agarose Gels for RNA Electrophoresis

Precast Agarose Gels for RNA Electrophoresis are suitable for separating RNA sizes from 0.25 to 10 kb.

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