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Sigma-Aldrich

Amino-terminal FLAG-BAP™ Fusion Protein

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MDL number:
MFCD00130921
NACRES:
NA.32

form

liquid

Quality Level

200

mol wt

~49 kDa

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

Amino-terminal FLAG-BAP™ Fusion Protein is a 467 amino acid N-terminal FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular mass of 49.3kDa. The N-terminal FLAG-BAP Fusion Protein migrates as a 45−55kDa band by SDS-PAGE depending on electrophoresis conditions. FLAG-BAP™ Fusion Protein has biotechnological applications and is used in the screening of recombinant fusion proteins.
The Amino-terminal FLAG-BAP Fusion Protein is a control prorein used to confirm the funtional integrity of the anti-FLAG M1 and M2 monoclonal antibodies in applications such as western blotting, immunoprecipitation, ELISA, electron microscopy, and FACS.

Application

Amino-terminal FLAG-BAP™ Fusion Protein has been used as a standard in enzyme-linked immunosorbent assay (ELISA) for osteopontin quantification and in western blotting based quantification of Flag-Wnt8 and Drosophila melanogaster transcription factor interactome.
Learn more product details in our FLAG® application portal.

Other Notes

Control protein

Physical form

Protein is supplied in 10 mM Tris, 120 mM NaCl,
0.05 mM ZnCl2 in 50% glycerol, pH 8.0.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Exploring the separation power of mixed-modal resins for purification of recombinant osteopontin from clarified Escherichia coli lysates
Guo S, et al.
Biotechnology Progress, 35(1), e2722-e2722 (2019)
Noggin4 is a long-range inhibitor of Wnt8 signalling that regulates head development in Xenopus laevis
Eroshkin FM, et al.
Scientific Reports, 6, 23049-23049 (2016)
BAP-fusion: A versatile molecular probe for biotechnology research
Biotechnology: Research, Technology and Applications, 327-345 (2008)
A Comprehensive Drosophila melanogaster Transcription Factor Interactome
Shokri L, et al.
Cell Reports, 27(3), 955-970 (2019)
Daniela Castiglia et al.
Biotechnology for biofuels, 9, 154-154 (2016-07-28)
Biofuels production from plant biomasses is a complex multi-step process with important economic burdens. Several biotechnological approaches have been pursued to reduce biofuels production costs. The aim of the present study was to explore the production in tobacco plastome of
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Anti-FLAG® M2 Magnetic Beads

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

Protocols

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

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