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PURE1A

Sigma-Aldrich

Protein A Antibody Purification Kit

sufficient for 10 purifications

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NACRES:
NA.32

usage

sufficient for 10 purifications

shipped in

wet ice

storage temp.

2-8°C

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General description

The Protein A Antibody Purification Kit provides all of the reagents necessary to isolate monoclonal and polyclonal antibodies.

Specificity

Capacity/Run (by Species)
Human IgG 20-25 mg
Mouse IgG1 8-12 mg
Mouse IgG2a 10-15 mg
Mouse IgG2b 6-10 mg
Mouse IgG3 15-20 mg
Rabbit IgG 8-12 mg
Goat IgG 2-4 mg
Bovine IgG 8-12 mg

Application

PURE-1A offers Protein A technology in a prepackaged, easy to use kit form. With this kit, milligram quantities of IgG can be purified from serum, ascites, or cell culture supernatants.
Purification of IgG from other species may be possible, however, the researcher will have to determine the suitability of the kit for their application.
The Protein A Antibody Purification Kit is suitable for use with IgGs from human, mouse, rabbit, goat, and bovine species.

Features and Benefits

  • High capacity - purify up to 8 mg of mouse IgG or 25 mg of human IgG per column run
  • Specific - will only bind IgG
  • Easy to use - antibody is eluted and desalted in a single step, ready to use
  • Gentle - avoids prolonged exposure of the antibody to low pH

Principle

Using our cartridge system, antibodies elute as highly purified proteins at physiological pH. Protein A is a powerful tool for isolation of antibodies from mammalian hosts. Protein A exhibits a high degree of specificity for IgG and ensures an antibody preparation virtually free of IgA, IgM and non-immunoglobulin serum proteins such as albumin.

Kit Components Only

Product No.
Description
  • Binding Buffer 225 mL
  • Desalting Cartridge 1 ea
  • Elution Buffer 75 mL
  • HEPES Buffer 225 mL
  • 5 ml Syringe 1
  • 10 ml Syringe 1
  • Protein A Cartridge 1 mL
  • Regeneration Buffer 75 mL
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Regulatory Information

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The venom proteome of the monocled cobra, Naja kaouthia, from Thailand, was characterized by RP-HPLC, SDS-PAGE, and MALDI-TOF-TOF analyses, yielding 38 different proteins that were either identified or assigned to families. Estimation of relative protein abundances revealed that venom is
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Penio T Todorov et al.
Cancer research, 67(23), 11419-11427 (2007-12-07)
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Hwang,G et al.
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Protocols

Antibody Purification using Protein A/G/L Agarose

Antibody purification protocols yield preparations containing endogenous IgG alongside specific antibodies.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service