QPBCA
QuantiPro™ BCA Assay Kit
for 0.5-30 μg/ml protein
shipped in
wet ice
storage temp.
2-8°C
Application
Features and Benefits
- Accurate across a broad range of protein concentrations
- High sensitivity; linear response form 0.5 to 30 μg/ml of protein
- Stable color complex
- Reduced susceptibility to detergents
Principle
Storage and Stability
Legal Information
Kit Components Only
- Copper(II) sulfate solution, 4 % (w/v) (prepared from copper (II) sulfate pentahydrate) 12 mL
Kit Components Also Available Separately
- P0914Protein Standard Solution: 1.0 mg/ml bovine serum albumin in 0.15 M NaCl with 0.05% sodium azide (flame-sealed glass ampules) 10 x 1SDS
Application
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2
Storage Class Code
8A - Combustible, corrosive hazardous materials
WGK
WGK 3
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Example:
Additional examples:
705578-5MG-PW
PL860-CGA/SHF-1EA
MMYOMAG-74K-13
1000309185
enter as 1.000309185)
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Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.
Aldrich Products
For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').
For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').
For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.
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Articles
This page describes common challenges encountered when lysing cells and extracting proteins prior to Western blotting. Total protein concentration must be determined for these cell lysates. Variables affecting each of these steps are outlined below, as each could affect the sensitivity and reproducibility of the Western blot.
The era of high-throughput proteomics has recently blossomed due in large part to advances in the methods by which proteins and proteomes are analyzed. Improved fractionation techniques, combined with advances in mass spectrometry, have decreased concerns of sample complexity, and directed more focus towards high-throughput techniques.
The possible causes and potential remedies for challenges encountered in the immunoprecipitation-Western blot technique, which consists of cell lysis, formation of the antibody-antigen (immune) complex, precipitation of the immune complexes, and analysis by Western blotting.
Related Content
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.
A wide variety of products for traditional protein quantitation techniques such as BCA and Bradford. Also, products for alternative assays such as Lowry, Micro Pyrogallol and FluoroProfile are also available for total protein determination.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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