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Merck
CN

S2890

Streptavidin−Alkaline Phosphatase from Streptomyces avidinii

lyophilized powder, ≥700 DEA units/mg protein

Synonym(s):

Streptavidin-AP, Streptavidin−AP from Streptomyces avidinii

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00132384

Key Documents

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Product Name

Streptavidin−Alkaline Phosphatase from Streptomyces avidinii, lyophilized powder, ≥700 DEA units/mg protein

conjugate

alkaline phosphatase conjugate

form

lyophilized powder

specific activity

≥700 DEA units/mg protein

composition

Protein, ≥40% biuret

storage temp.

−20°C

Quality Level

200

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Application

Streptavidin-alkaline phosphatase from Streptomyces avidinii has been used in enzyme-linked immune absorbent spot (ELISpot) to detect interferon (IFN-γ) producing cells.
The optimal working dilution should be determined empirically using a range of dilutions from a 1 mg/ml stock in buffer.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Streptavidin, a 58 kDa protein, is a homotetramer purified from Streptomyces avidinii. It has a high affinity for biotin. Streptavidin has been conjugated with either peroxidase or alkaline phosphatase (ALP). These enzymes are used as protein conjugates because of their comparative stability and a wide range of suitable substrates for different applications. ALP conjugates to streptavidin in immunoassays.

Other Notes

One alkaline phosphatase unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.

Physical form

Lyophilized powder containing trehalose with EPPS, phosphate and traces of magnesium chloride and zinc chloride.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
SLCN4419
SLCN4419
SLCL2931
SLCL2931
SLCF8013

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Sialoside specificity of the siglec family assessed using novel multivalent probes: identification of potent inhibitors of myelin-associated glycoprotein.
Blixt O et al
The Journal of Biological Chemistry, 278(33), 31007-31019 (2003)
Matured tolerogenic dendritic cells effectively inhibit autoantigen specific CD4+ T cells in a murine arthritis model
Jansen MAA, et al.
Frontiers in Immunology, 10, 2068-2068 (2019)
Effects of bovine Herpesvirus Type 5 on development of in vitro-produced bovine embryos.
Silva-Frade C
Theriogenology, 73(3), 324-331 (2010)
Microbial Resource Conservation: Conventional to Modern Approaches (2018)
Ola Blixt et al.
The Journal of biological chemistry, 278(33), 31007-31019 (2003-05-30)
Ten of the 11 known human siglecs or their murine orthologs have been evaluated for their specificity for over 25 synthetic sialosides representing most of the major sequences terminating carbohydrate groups of glycoproteins and glycolipids. Analysis has been performed using
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Protocols

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

二乙醇胺测定法用于碱性磷酸酶的酶法测定（EC 3.1.3.1）

碱性磷酸酶的酶法测定，二乙醇胺法（EC 3.1.3.1)

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