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Sigma-Aldrich

Enhanced Avian First Strand Synthesis Kit

Components for cDNA synthesis with enhanced AMV reverse transcriptase

Synonym(s):

Reverse Transcriptase cDNA synthesis kit, eAMV-RT

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.55

Key Documents

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Quality Level

200

usage

sufficient for 50 reactions
 kit sufficient for 50 reactions

feature

dNTPs included
hotstart: no

technique(s)

RT-PCR: suitable

color

colorless

input

purified RNA

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

Enhanced Avian First Strand Synthesis Kit utilizes a highly purified avian myeloblastosis virus reverse transcriptase (eAMV-RT) that offers superior performance in comparison to standard AMV-RT or standard Moloney murine leukemia virus reverse transcriptase (MMLV-RT). This exceptionally robust eAMV-RT has an enhanced ability to transcribe through difficult secondary structure at elevated temperatures (up to 65 °C) making it the ideal enzyme for producing high quality full-length cDNA from total RNA or poly(A)+ RNA.
Sigma′s Enhanced Avian RT First Strand Synthesis Kit also provides random nonamers (9-mers) and anchored oligo (dT)23 primers since gene-specific primers may not always be useful or possible. The anchored oligo has 23 thymidine residues and one G, C, or A residue (the anchor). The anchor ensures that the oligo (dT) primer binds at the very beginning of the message and that there is not a long region of the unusable sequence. With this kit, a dependable cDNA is generated that can be used for various downstream applications, including PCR.

Application

Enhanced Avian First Strand Synthesis Kit has been used for cDNA synthesis, which is intended for use in a real-time reverse transcription-polymerase chain reaction (RT-PCR).

Other Notes

One unit incorporates one nanomole of TMP into TCA-precipitable material in 10 minutes using polyadenylic acid as template and oligo(dT)12-18 as primer.

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Kit Components Only

Product No.
Description
  • Enhanced Avian Reverse Transcriptase 1000 U

Kit Components Also Available Separately

Product No.
Description
SDS
  • Deoxynucleotide mix 50 μL
  • O4387Anchored oligo (dT)23 100 μLSDS
  • R7647Random nonamers 100 μLSDS
  • O4387Ribonuclease inhibitor 50 μLSDS
  • W1754PCR grade water 1.5 mLSDS

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

监管及禁止进口产品
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Certificates of Analysis (COA)

Lot/Batch Number
SLCQ9453
SLCP5967
SLCN0255
SLCH8162
SLCH2897

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Sigma-Aldrich Corporation's Life Science Quarterly. Hot Start RT-PCR results in improved performance of the enhanced Avian RT-PCR
Easlund, E., and Mueller, E.
Sigma data, 2-5 (2001)
María José Martín et al.
The Journal of biological chemistry, 282(47), 34510-34524 (2007-09-21)
Heme oxygenase-1 (HO-1), an inducible enzyme that metabolizes the heme group, is highly expressed in human Kaposi sarcoma lesions. Its expression is up-regulated by the G protein-coupled receptor from the Kaposi sarcoma-associated herpes virus (vGPCR). Although recent evidence shows that
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Journal of leukocyte biology, 82(6), 1554-1563 (2007-11-28)
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Articles

Reverse Transcription

Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.

逆转录

分析基因表达的方法之一是测量基因的mRNA浓度。此类分析面临着若干挑战，例如不同转录本之间的半衰期不同、转录时间模式以及mRNA和蛋白质之间缺乏相关性。

Protocols

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

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