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T1323

Sigma-Aldrich

Monoclonal Anti-ε-Tubulin antibody produced in mouse

clone TUB-11, purified immunoglobulin, buffered aqueous solution

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MDL number:
MFCD03792420
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

TUB-11, monoclonal

form

buffered aqueous solution

mol wt

antigen 55 kDa

species reactivity

frog, mouse, chicken, human

technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 2-4 μg/mL using whole extract of cultured chicken fibroblasts

isotype

IgG2a

UniProt accession no.

Q9UJT0

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBE1(51175)
mouse ... Tube1(71924)

General description

Monoclonal Anti-ε-Tubulin (mouse IgG2a isotype) is derived from the TUB-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a synthetic peptide.
Tubulin epsilon 1 (ε-tubulin) is a member of tubulin superfamily. ε-tubulin is localized to the centrosome in cell-cycle-dependent manner. It is encoded by the gene mapped to human chromosome 6q21.

Specificity

Monoclonal Anti-ε-Tubulin recognizes human, mouse, frog, and chicken ε-tubulin (55 kDa).

Immunogen

synthetic peptide corresponding to amino acids 352-366 of human ε-tubulin, conjugated to KLH.

Application

Monoclonal Anti-ε-Tubulin antibody produced in mouse may be used in:
  • immunoblotting
  • enzyme-linked immunosorbent assay (ELISA)
  • immunofluorescence

Biochem/physiol Actions

Tubulin ε 1 (ε-Tubulin) plays a vital role in microtubule anchoring and centriole maturation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at−20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Regulatory Information

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Centrosome composition and microtubule anchoring mechanisms
Bornens M.
Current Opinion in Cell Biology, 14, 25-34 (2002)
ε-Tubulin Is an Essential Component of the Centriole
Dutcher SK
Molecular Biology of the Cell, 13, 3859?3869-3859?3869 (2002)
Paul Chang et al.
Nature cell biology, 5(1), 71-76 (2003-01-03)
Centrosomes nucleate microtubules and serve as poles of the mitotic spindle. Centrioles are a core component of centrosomes and duplicate once per cell cycle. We previously identified epsilon-tubulin as a new member of the tubulin superfamily that localizes asymmetrically to
P G McKean et al.
Journal of cell science, 114(Pt 15), 2723-2733 (2001-10-31)
Although most eukaryotic cells can express multiple isotypes of alphabeta-tubulin, the significance of this diversity has not always been apparent. Recent data indicate that particular alphabeta-tubulin isotypes, both genome encoded and those derived by post-translational modification, can directly influence microtubule
Nafees Ahmad et al.
The International journal of developmental biology, 57(9-10), 741-751 (2013-12-07)
Pitx3 is a bicoid-related homeodomain transcription factor critical for the development of the ocular lens, mesencephalic dopaminergic neurons and skeletal muscle. In humans, mutations in PITX3 are responsible for cataracts and anterior segment abnormalities of varying degree; polymorphisms are associated

Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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