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W1754

Water

Name: Water
Brand: SIGMA

PCR Reagent, suitable for PCR

Synonym(s):

H₂O

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About This Item

Linear Formula:

H₂O

CAS Number:

7732-18-5

Molecular Weight:

18.02

NACRES:

NA.25

PubChem Substance ID:

24900779

UNSPSC Code:

12191602

EC Number:

231-791-2

MDL number:

MFCD00011332

Beilstein/REAXYS Number:

2050024

Technique(s):

PCR: suitable

Bp:

100 °C (lit.)

Vapor pressure:

3 mmHg

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Product Name

Water, PCR Reagent

vapor density

<1 (vs air)

Quality Level

200

vapor pressure

3 mmHg

sterility

sterile-filtered

form

liquid

packaging

vial of 1.5 mL

technique(s)

PCR: suitable

refractive index

n20/D 1.34 (lit.)

pH

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

foreign activity

DNase, none detected, RNase, none detected

SMILES string

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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Analytical Chemistry

Analytical Chromatography

Lab Chemicals

Solvents

General description

PCR grade water is sterile-filtered. It is free from exonucleases (DNAse, RNAse) and endonuclease (NICKase) and is also free from nucleic acid contamination.

Application

Water has been used:

as a component of the reaction mixture and as a diluting agent in microfluidic RT-qPCR
as a component of the reaction mixture for the amplification of products from fungal (Trametes versicolor) DNA
as a diluting agent and as a component of the reaction mixture for the amplification of cDNA
Water has been used to make up the final volume of the sample in polymerase chain reaction (PCR)

Suitable for polymerase chain reaction (PCR)

Other Notes

Easily compare specifications for Water products with the Water specification table.

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases.

Matthew J Ellington et al.

The Journal of antimicrobial chemotherapy, 59(2), 321-322 (2006-12-23)

Detection of legionellae in hospital water samples by quantitative real-time LightCycler PCR.

N Wellinghausen et al.

Applied and environmental microbiology, 67(9), 3985-3993 (2001-08-30)

Contamination of hospital water systems with legionellae is a well-known cause of nosocomial legionellosis. We describe a new real-time LightCycler PCR assay for quantitative determination of legionellae in potable water samples. Primers that amplify both a 386-bp fragment of the

Incidence and survival of non-O157 verocytotoxigenic Escherichia coli in soil

Bolton DJ, et al.

Journal of Applied Microbiology, 111(2), 484-490 (2011)

Identification of Naegleria fowleri in domestic water sources by nested PCR.

Francine Marciano-Cabral et al.

Applied and environmental microbiology, 69(10), 5864-5869 (2003-10-09)

The free-living amoeboflagellate Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis (PAM), a rapidly fatal disease of the central nervous system. In the United States, the disease is generally acquired while swimming and diving in freshwater lakes and

Circulating Tfh1 (cTfh1) cell numbers and PD1 expression are elevated in low-grade B-cell non-Hodgkin?s lymphoma and cTfh gene expression is perturbed in marginal zone lymphoma

Elliot T B, et al.

PLoS ONE (2018)

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Antibody-Enzyme Mediated Hot Start PCR Protocol

JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.

Hot Start dNTP Protocol

Hot start dNTP protocol enhances specificity in PCR by blocking DNA polymerase nucleotide incorporation during PCR.

Amplification of Genomic DNA using REDTaq® DNA Polymerase

Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.

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Global Trade Item Number

SKU	GTIN
W1754-5VL	04061837488696
W1754-1VL	04061837488689

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