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Z722553

ThermalSeal RT2RR™ film

non-sterile, sealing films for real-time qPCR

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Synonym(s):
ThermalSeal RT™ film, real-time qPCR plate seal
NACRES:
NB.22

material

polyester film

sterility

non-sterile

packaging

pack of 100 ea

manufacturer/tradename

Excel Scientific TS-RT2RR-100

technique(s)

PCR: suitable
qPCR: suitable

L

130.6 mm

W × L

7.78 cm × 13.08 cm

W

77.8 mm

color

clear

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General description

A combination of optically transparent polyester film with a strong, ultra-smooth, non-absorbing, non-fluorescing medical-grade adhesive for superior performance in real-time PCR applications. In place of the customary paper backing, ThermalSeal RT has a plastic liner which is easily removed before use and contributes smoothness and extreme optical clarity to the adhesive. Dimensions are 3.125 x 5.625 in. to seal PCR plates. Length between the perforated ends is 4.8 in.
  • Ultra-high optical clarity
  • Heat resistant, recommended for temperatures from -40°C to +120°C
  • Certified DNase, RNase, and nucleic acid free
ThermalSeal RT2RR™ 50 μm polyester sealing films for real-time PCR are sized to fit within the edges of raised-rim 96 well plates. The same consistent ultra-high optical clarity as Excel′s ThermalSeal RT™ sealing films makes possible more reproducible, reliable, and consistent DNA amplification measurements. An inert, strong, temperature-resistant adhesive assures reliable sealing around each well. Two end tabs assist in positioning the sealing film. Easy removal of the end tabs at perforated boundaries prevents lifting and higher evaporation rates that can occur with sealing films that overlap the plate rim. Recommended for specific DNA sequence detection in clinical diagnostics, forensic science and basic research.
Dimensions 77.8 by 130.8 mm. With end tabs removed, length is 118.1 mm with 45° corners.
  • Ultra-high optical clarity
  • Fit within plate rim to prevent evaporation due to sealing film lifting
  • Heat resistant, recommended for temperatures from
  • -40 °C to +120 °C
  • Certified DNase, RNase, and Nucleic Acid free

Legal Information

ThermalSeal is a registered trademark of Excel Scientific, Inc.
ThermalSeal RT is a trademark of Excel Scientific, Inc.
ThermalSeal RT2RR is a trademark of Excel Scientific, Inc.

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Protocols

Reverse Transcription Protocol Using SYBR Green Dye Detection

Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.

Multiplex qPCR Protocol

Standard qPCR protocol with up to four detection probes at specified concentrations, simplifying reaction setup with LuminoCt ReadyMix.

qPCR with Single Detection Probe

qPCR protocol template with specific detection probes aids in single-target detection using LuminoCt® ReadyMix™.

KiCqStart™ Universal SYBR® Green qPCR Protocol

Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.

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