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Merck
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BS-41601Z

Sigma-Aldrich

GlycoProfile EZGlyco® O-Glycan Prep Kit

Chemical O-glycan Release

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About This Item

UNSPSC Code:
12352204
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conjugate

(O-linked)

technique(s)

HPLC: suitable
mass spectrometry (MS): suitable

General description

  • The kit adopts a unique O-glycan releasing method “Eliminative Oximation” using a high reactive amine and an organic superbase.
  • Applications include characterization of O-glycosylation of recombinant glycoproteins in upstream R&D, quality control and process control.
  • What’s included:
    • Protocol
    • Glycan Release Reagent A
    • Glycan Release Reagent B
    • Glycan Capturing Beads
    • Filter Column, 2-Aminobezamide
    • Reducing Reagent
    • Cleanup Column

Application

  • Useful for release and labeling of O-linked glycans from glycoproteins for Glycomics by Mass Spec, capillary electrophoresis, HPLC or other analysis methods.
  • O-glycan biomarker discovery and screening in biological samples such as mucins, serum and exosomes
  • O-glycan analysis of functional foods such as dairy products

Features and Benefits

O-Glycan sample prep in 5 hours

High recovery

Minimize "peeling"

Easy and safe operation

Legal Information

EZGlyco is a registered trademark of SUMITOMO BAKELITE CO., LTD.
GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 3 - Carc. 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT RE 2 - STOT SE 3 - Water-react 2

Target Organs

Blood, Respiratory system

Storage Class Code

4.3 - Hazardous materials which set free flammable gases upon contact with water

WGK

WGK 3

Regulatory Information

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Articles

Learn about O-linked glycan strategies, O-glycosidase actions, how to remove sialic acid residues, β-Elimination, and O-glycan modifications.

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