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About This Item
UNSPSC Code:
41106514
Biological source:
hamster embryo
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Fibroblast
biological source
hamster embryo
packaging
tube of 5 μg 00030814-DNA-5UG, pkg of vial of cells 00030814-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
PAR-1 (protease-activated receptor 1, thrombin receptor), EGF, PDGF
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
IIC9 (CH0) is suitable for selective growth of some rodent epithelial cells in a medium containing citrulline.
Biochem/physiol Actions
Chinese hamster embryo fibroblast
The Chinese hamster embryo fibroblast (CHEF) line is a nontumorigenic and stable diploid cell line, with a high serum requirement for growth. It is isolated from embryo preparation and is a spontaneous tumorigenic anchorage-independent line.
Preparation Note
Split sub-confluent cultures (70-80%) 1:10 ie. seeding at 2-4 x10,000 cells/cm2 using 0.25% trypsin /EDTA; 37°C; 5% CO2.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Regulatory Information
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