packaging
tube of 5 μg 01080924-DNA-5UG, pkg of vial of cells 01080924-1VL
ImmunoDon
BALB/c mice
Myeloma
Ag876
Reactivity
Anti-Osteoclasts (human foetal), ECV304 and U937 cells
technique(s)
cell culture | mammalian: suitable
isotype
IgM plus IgG
shipped in
dry ice
storage temp.
−196°C
Immunogen
Osteoclastoma tumour
Application
Immunocytochemistry
Biochem/physiol Actions
135B hybridoma was generated by fusing Ag876 myeloma cells with splenic cells of BALB/c mice immunised with tartrate-resistant acid phosphatase (TRAP) isolated from human osteoclastoma tumour. V35B antibody shows reactivity against the monocytic leukemia cell line U937, ECV304 cells and guinea pig tissue.
Anti-Osteoclastoma Hybridoma
Preparation Note
Allow hybridomas to become 75% confluent prior to splitting. Seed at 3-9 x 100,000 cells/ml 5% CO2, 37°C,
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.