JB1
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
packaging
tube of 5 μg 01091808-DNA-5UG
pkg of vial of cells 01091808-1VL
ImmunoDon
BALB/c B27 female transgenic mouse
Myeloma
PS-NS1/Ag 4.1
Reactivity
Anti- aggregated HFE protein, Human
technique(s)
cell culture | mammalian: suitable
isotype
IgG3
shipped in
dry ice
storage temp.
−196°C
Immunogen
Bacterial recombinant HFE protein
Biochem/physiol Actions
Anti-recombinant HFE Protein hybridoma
To produce this hybridoma mice transgenic HLA-B27 and human β-2 microglobulin were immunized with a preparation of HFE made in recombinant bacteria, refolded with human β-2 microglobulin. Characterisation beyond data in B.J. Haematol 103, 931, 1998 shows that JB1 reacts predominantly with aggregated denatured HFE produced in bacteria, but does not bind folded recombinant HFE produced in CHO cells. In comparison with polyclonal sera raised to the C terminus or the α 3 region of HFE JB1 stains frozen sections of the tissues well, but reacts very weakly with cells transfected with the HFE gene in vitro (usually only visible by confocal microscopy). JB1 does not stain paraffin fixed sections and will not work in western blots.
Preparation Note
Maintain cells at 3-5x100,000 cells/ml which is usually a split ratio of 1:2. Saturation density os 5 x 100,000 to 1 x 1000,000.
RPMI 1640, 10% FBS, 1% HAT
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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