biological source
rat lung
packaging
tube of 5 μg 04100601-DNA-5UG
pkg of vial of cells 04100601-1VL
growth mode
Adherent
karyotype
Hypotetraploid, n=74 chromosomes (median)
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat Bronchial Epithelial Cells (RaBE)
Cell Line Description
Spontaneously immortalised rat bronchial epithelial cell line derived from a 4 month old male Wistar rat. The cell line is basal cell-like expressing cytokeratins 14 and 15. Vimentin negative. A slow growing derivative of RaBE, cultured in the absence of growth factors is also available - RaBE (Slow Growth), Sigma Catalogue number. 04100602.
Culture Medium
Ham′s F12 + 2mM Glutamine + 10ug/ml transferrin + 5ug/ml insulin + 10ng/ml EGF + 10ng/ml aFGF + 100ng/ml cholera toxin + 5% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) between 1:100 and 1:800 i.e. seeding 5000 cells/25cm2 with 5ml or 10,000 cells/75cm2 with 20ml using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Subculture once a week, double volume
Other Notes
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