biological source
human bladder
packaging
tube of 5 μg 06013102-DNA-5UG, pkg of vial of cells 06013102-1VL
growth mode
Not specified
karyotype
Aneuploid, with specific marker chromosomes
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Human bladder transitional cell carcinoma
Spontaneously immortal cell line established from a primary transitional cell carcinoma of the bladder. Derived from a female patient.
Preparation Note
2/3 DMEM:1/3 RPMI + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. The medium must be changed to fresh medium every 48 hours.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.