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UNSPSC Code:
41106514
Biological source:
human bone marrow
Relevant disease(s):
cancer
Growth mode:
Suspension
Karyotype:
Hyperdiploid with complex aberrant chromosomes
Morphology:
Round to polygonal cells growing in clusters in suspension
biological source
human bone marrow
packaging
tube of 5 μg 06072606-DNA-5UG, pkg of vial of cells 06072606-1VL
growth mode
Suspension
karyotype
Hyperdiploid with complex aberrant chromosomes
morphology
Round to polygonal cells growing in clusters in suspension
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Biochem/physiol Actions
Established in 1984 from the bone marrow of a 67-year old woman diagnosed with myeloma who survived for one year after diagnosis.
Human multiple myeloma
Not specified
Preparation Note
Doubling time approximately 30 hours. Cells grow singly in suspension. Maintain cultures between 5-20 x 105 cells/ml; 5% CO2; 37 °C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 5 x 105 cells/ml. At subculture perform a cell count and maintain cultures between 5-20 x 105 cells/ml, this tends to equate to a 1:3 split every 2-3 days.
RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS)
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.