KARPAS-929
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Synonym(s):
KARPAS 929, KARPAS, 929, KARPAS929
biological source
human bone marrow
packaging
tube of 5 μg 06072606-DNA-5UG
pkg of vial of cells 06072606-1VL
growth mode
Suspension
karyotype
Hyperdiploid with complex aberrant chromosomes
morphology
Round to polygonal cells growing in clusters in suspension
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Biochem/physiol Actions
Established in 1984 from the bone marrow of a 67-year old woman diagnosed with myeloma who survived for one year after diagnosis.
Human multiple myeloma
Not specified
Preparation Note
Doubling time approximately 30 hours. Cells grow singly in suspension. Maintain cultures between 5-20 x 105 cells/ml; 5% CO2; 37 °C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 5 x 105 cells/ml. At subculture perform a cell count and maintain cultures between 5-20 x 105 cells/ml, this tends to equate to a 1:3 split every 2-3 days.
RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS)
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Regulatory Information
监管及禁止进口产品
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