biological source
human blood (peripheral)
packaging
tube of 5 μg 06072607-DNA-5UG, pkg of vial of cells 06072607-1VL
growth mode
Aggregates in suspension
karyotype
Refer to Nacheva et al., 1994
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
A human B cell lymphoma established from a pleural effusion of a 23-year-old woman with mediastinal lymphoblastic B-cell non-Hodgkin′s lymphoma. This cell line does not express BCL2.
Human B-Cell Non-Hodgkin′s Lymphoma
Preparation Note
RPMI 1640 + 2mM Glutamine + 10 - 20% Foetal Bovine Serum (FBS)
Maintain cultures between 0.5 - 1.5 x1000,000 cells/ml; 5% CO2; 37°C. Doubling time approximately 60-100 hours. Split saturated culture 1:2 to 1:3 every 2-3 days. Spherical cells growing in clusters in suspension. This cell line can be grown with 10% Foetal Bovine Serum rather than 20%.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.