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About This Item
UNSPSC Code:
41106514
Biological source:
human blood (peripheral)
Relevant disease(s):
cancer
Growth mode:
Aggregates in suspension
Karyotype:
Refer to Nacheva et al., 1994
Morphology:
Lymphoblastoid
biological source
human blood (peripheral)
packaging
tube of 5 μg 06072607-DNA-5UG, pkg of vial of cells 06072607-1VL
growth mode
Aggregates in suspension
karyotype
Refer to Nacheva et al., 1994
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
A human B cell lymphoma established from a pleural effusion of a 23-year-old woman with mediastinal lymphoblastic B-cell non-Hodgkin′s lymphoma. This cell line does not express BCL2.
Human B-Cell Non-Hodgkin′s Lymphoma
Preparation Note
Maintain cultures between 0.5 - 1.5 x1000,000 cells/ml; 5% CO2; 37°C. Doubling time approximately 60-100 hours. Split saturated culture 1:2 to 1:3 every 2-3 days. Spherical cells growing in clusters in suspension. This cell line can be grown with 10% Foetal Bovine Serum rather than 20%.
RPMI 1640 + 2mM Glutamine + 10 - 20% Foetal Bovine Serum (FBS)
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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