NS20Y-BU-2
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
mouse nerve
packaging
tube of 5 μg 08062502-DNA-5UG
pkg of vial of cells 08062502-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Neuronal
products
Choline acetyltransferase
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Used in the generation of cell hybrids.
Biochem/physiol Actions
A bromodeoxyuridine-resistant mutant of NS20Y (Sigma Catalogue number 08062517) The cell line NS20Y is a cholinergic cell line derived from mouse neuroblastoma C1300 Mouse strain A/Jax.
Mouse neuroblastoma
Preparation Note
DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)+ 1 mM 8-bromodeoxyuridine. Cells can also be grown in the absence of bromodeoxyuridine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
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