Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
UNSPSC Code:
41106514
Biological source:
human eye
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Polygonal
biological source
human eye
packaging
tube of 5 μg 09061602-DNA-5UG, pkg of vial of cells 09061602-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Polygonal
products
Cytokeratins 5 and 8, junctional protein zonula occludens (ZO-1)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
Biochem/physiol Actions
An immortalized retinal pigment epithelial cell line
STR-PCR Data:
Amelogenin: X
CSF1PO: 10,13
D13S317: 12
D16S539: 12
D5S818: 9,13
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 16,19
Amelogenin: X
CSF1PO: 10,13
D13S317: 12
D16S539: 12
D5S818: 9,13
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 16,19
The h1RPE7 cell line was derived from primary human retinal pigment epithelial cells obtained from a 50-year-old female Caucasian donor. The primary culture was transfected with a construct encoding SV40 large T antigen that was deleted for the small t antigen along with puromycin resistance gene.
Preparation Note
Hams F10 + 20% Heat-inactivated FCS + 2 mM Glutamine + selection antibiotic 1μg/ml puromycin.
Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 3 x 10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Population doubling approx 24hrs.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Regulatory Information
新产品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service