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About This Item
UNSPSC Code:
41106514
Biological source:
human colon
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Near diploid
Morphology:
Epithelial
biological source
human colon
packaging
tube of 5 μg 09071501-DNA-5UG, pkg of vial of cells 09071501-1VL
growth mode
Adherent
karyotype
Near diploid
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Human colorectal adenocarcinoma
Not specified
Subpopulations isolated from the HCA-7 cell line following butyrate treatment.
Preparation Note
DMEM (High glucose Sigma D6546) + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:5 to 1:10 weekly using 0.05% trypsin/EDTA; 7.5% CO2; 37 °C. Suggested seeding density 2-4x10,000 cells/cm2.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Regulatory Information
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