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About This Item
UNSPSC Code:
41106514
Biological source:
human pancreas (islets)
Growth mode:
Adherent
Karyotype:
Modal chromosome number 67-71
Morphology:
Epitheloid
biological source
human pancreas (islets)
packaging
tube of 5 μg 10070103-DNA-5UG, pkg of vial of cells 10070103-1VL
growth mode
Adherent
karyotype
Modal chromosome number 67-71
morphology
Epitheloid
products
1.2B4 cells have been shown to express insulin, glucokinase and IAPP by immunocytochemistry. These cells express the GLUT1 glucose transporter.
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
Biochem/physiol Actions
1.2B4 is a hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with HuP-T3, a human pancreatic carcinoma cell line (Sigma catalog number 93121055). 1.2B4 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic cell biology. 1.2B4 cells provide a method of producing pure insulin secreting cells. This is an alternative to the use of primary tissue in cell transplantation therapies for type 1 diabetes.
STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 10
D13S317: 9,11
D16S539: 10,13
D5S818: 10,14
D7S820: 12
THO1: 9
TPOX: 8,12
vWA: 18
Amelogenin: X,Y
CSF1PO: 10
D13S317: 9,11
D16S539: 10,13
D5S818: 10,14
D7S820: 12
THO1: 9
TPOX: 8,12
vWA: 18
Preparation Note
RPMI-1640 + 2 mM Glutamine + 10% FCS / FBS
Split sub-confluent cultures (70-80%) 1:2 to 1:6 , i.e., seeding at 2-4 x 104 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Population doubling approx 20hrs. At confluence 105 cells/cm2 can be expected.
Other Notes
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Legal Information
Flatt P & McClenaghan N (2004) Insulin producing cell-line US patent number 20040005702 (A1). Also WO0210346 (A2).
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Regulatory Information
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