biological source
Human x Mouse Hybridoma
packaging
tube of 5 μg 10080903-DNA-5UG, pkg of vial of cells 10080903-1VL
ImmunoDon
Mouse (Mus musculus) spleen
Myeloma
PAI
Reactivity
Human CD120a (TNF receptor p55)
technique(s)
cell culture | mammalian: suitable
isotype
IgG2b
shipped in
dry ice
storage temp.
−196°C
Immunogen
Human CD120a (TNF receptor p55)
Application
Sandwich ELISA for soluble p55 TNF-R.
Biochem/physiol Actions
Not specified
Preparation Note
Iscoves Modified Dulbeccos Medium (IMDM) + 2 mM glutamine + 25 U/ml Interleukin-6 (IL-6) + 5-10% Fetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA maybe required to dislodge them from the plastic.
Seed cultures between 1-3 x 105 cells/ml; Saturation density 8-10 x 105 5% CO2; 37 °C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.