biological source
Human x Mouse Hybridoma
packaging
tube of 5 μg 10080904-DNA-5UG, pkg of vial of cells 10080904-1VL
ImmunoDon
Mouse (Mus musculus) spleen
Myeloma
PAI
Reactivity
Human CD120b (TNF receptor p75)
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Application
Sandwich ELISA for soluble p75 TNF-R. No competition with TNF.
Preparation Note
Iscoves Modified Dulbeccos Medium (IMDM) + 2 mM glutamine + 25 U/ml Interleukin-6 (IL-6) + 5-10% Fetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA may be required to dislodge them from the plastic.
Seed cultures between 1-3 x 105 cells/ml; Saturation density 8-10 x 105 5% CO2; 37 °C
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.